PMID- 1423884
OWN - NLM
STAT- MEDLINE
DCOM- 19921222
LR  - 20190509
IS  - 0143-3334 (Print)
IS  - 0143-3334 (Linking)
VI  - 13
IP  - 11
DP  - 1992 Nov
TI  - Proliferative dependent regulation of the glyceraldehyde-3-phosphate 
      dehydrogenase/uracil DNA glycosylase gene in human cells.
PG  - 2127-32
AB  - The relationship between the proliferative dependent expression of the 
      glyceraldehyde-3-phosphate dehydrogenase (GAPDH)/uracil DNA glycosylase (UDG) 
      gene and the induction of uracil DNA glycosylase activity was examined in human 
      cells. Three different cell types were studied to determine whether the 
      growth-dependent regulation of this multifunctional gene was a common 
      characteristic of human cells. These included WI-38 normal embryonic lung 
      fibroblasts, a Japanese Bloom's syndrome non-transformed skin fibroblast cell 
      strain (GM-05289) and a lymphoblastoid cell line transformed by the Epstein-Barr 
      virus. The Japanese Bloom's syndrome cells displayed the altered immunoreactivity 
      with marker monoclonal antibody 40.10.09 which characterizes cells from this 
      human genetic disorder. In noncycling human cells Northern blot analysis using a 
      plasmid (pChug 20.1) which contained the human GAPDH/UDG cDNA revealed a single 
      1.6 kb transcript. In each case, the expression of this gene was increased during 
      cell proliferation. This increase in GAPDH/UDG gene expression was identical to 
      that observed for UDG enzyme activity. Further, using anti-human UDG monoclonal 
      antibodies, there was a growth-dependent rise in immunoreactivity suggesting an 
      increase in the level of antigenic protein. These results demonstrate that: (i) 
      the expression of the GAPDH/UDG gene was dependent on the proliferative state of 
      the cell; and (ii) a correlation existed between the transcription of this gene 
      and the level of uracil DNA glycosylase enzyme activity.
FAU - Meyer-Siegler, K
AU  - Meyer-Siegler K
AD  - Fels Institute for Cancer Research and Molecular Biology, Philadelphia, PA.
FAU - Rahman-Mansur, N
AU  - Rahman-Mansur N
FAU - Wurzer, J C
AU  - Wurzer JC
FAU - Sirover, M A
AU  - Sirover MA
LA  - eng
GR  - CA-12227/CA/NCI NIH HHS/United States
GR  - CA-29414/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Carcinogenesis
JT  - Carcinogenesis
JID - 8008055
RN  - EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases)
RN  - EC 3.2.2.- (DNA Glycosylases)
RN  - EC 3.2.2.- (N-Glycosyl Hydrolases)
RN  - EC 3.2.2.- (Uracil-DNA Glycosidase)
SB  - IM
GS  - GAPDH
GS  - UDG
MH  - Blotting, Northern
MH  - *Cell Division
MH  - Cell Transformation, Viral
MH  - Cells, Cultured
MH  - *DNA Glycosylases
MH  - *DNA Repair
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Fibroblasts/enzymology
MH  - *Gene Expression Regulation, Enzymologic
MH  - Glyceraldehyde-3-Phosphate Dehydrogenases/*genetics/metabolism
MH  - Humans
MH  - N-Glycosyl Hydrolases/*genetics/metabolism
MH  - Transcription, Genetic
MH  - Uracil-DNA Glycosidase
EDAT- 1992/11/01 00:00
MHDA- 1992/11/01 00:01
CRDT- 1992/11/01 00:00
PHST- 1992/11/01 00:00 [pubmed]
PHST- 1992/11/01 00:01 [medline]
PHST- 1992/11/01 00:00 [entrez]
AID - 10.1093/carcin/13.11.2127 [doi]
PST - ppublish
SO  - Carcinogenesis. 1992 Nov;13(11):2127-32. doi: 10.1093/carcin/13.11.2127.