PMID- 1423922
OWN - NLM
STAT- MEDLINE
DCOM- 19921211
LR  - 20061115
IS  - 0092-6213 (Print)
IS  - 0092-6213 (Linking)
VI  - 38
IP  - 2
DP  - 1992 Oct
TI  - Production of tumor necrosis factor-alpha and interleukin-6 in mice infected with 
      group B streptococci.
PG  - 138-44
AB  - Group B streptococci (GBS) are a leading cause of sepsis and meningitis in 
      neonates. Since cytokines are thought to play an important role in septic shock, 
      we have studied serum levels of tumor necrosis factor-alpha (TNF alpha) and 
      interleukin-6 (IL-6) in BALB/c mice infected with type III GBS. TNF alpha and 
      IL-6 were detected by the L929 cytotoxicity and the B9 proliferation assays, 
      respectively, in serial serum samples obtained after infection. After i.p. 
      challenge with an LD50, serum TNF alpha rose above baseline values as early as 3 
      hr, peaked at 7 hr, and returned to baseline values at 20 hr. IL-6 serum levels 
      rose concomitantly with TNF alpha, peaking 8 hr after challenge. No serum TNF 
      alpha activity was detected in the course of sublethal infections. However, a 
      transient rise in TNF alpha levels was observed after i.v. inoculation of high 
      numbers (greater than or equal to 1 x 10(8) of heat-killed GBS. When groups of 
      mice were injected i.v. with a single dose of anti-TNF alpha rabbit serum 2 hr 
      before challenge with an LD90 or LD30, no effect was noted in terms of survival, 
      although the serum TNF alpha peak was completely abrogated. Serum TNF alpha does 
      not seem to play an obligatory role in GBS-induced lethality of adult mice. 
      However, further studies are needed to assess better the role of this cytokine in 
      the pathogenesis of GBS sepsis.
FAU - Teti, G
AU  - Teti G
AD  - Istituto di Microbiologia, Facolta di Medicina, Universita di Messina, Italy.
FAU - Mancuso, G
AU  - Mancuso G
FAU - Tomasello, F
AU  - Tomasello F
FAU - Chiofalo, M S
AU  - Chiofalo MS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Circ Shock
JT  - Circulatory shock
JID - 0414112
RN  - 0 (Antibodies)
RN  - 0 (Interleukin-6)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Antibodies/administration & dosage
MH  - Female
MH  - Interleukin-6/*immunology
MH  - Meningitis/immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Sepsis/immunology
MH  - Streptococcal Infections/*immunology
MH  - Streptococcus agalactiae/*immunology/pathogenicity
MH  - Tumor Necrosis Factor-alpha/*immunology
EDAT- 1992/10/01 00:00
MHDA- 1992/10/01 00:01
CRDT- 1992/10/01 00:00
PHST- 1992/10/01 00:00 [pubmed]
PHST- 1992/10/01 00:01 [medline]
PHST- 1992/10/01 00:00 [entrez]
PST - ppublish
SO  - Circ Shock. 1992 Oct;38(2):138-44.