PMID- 1425412
OWN - NLM
STAT- MEDLINE
DCOM- 19921201
LR  - 20061115
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 131
IP  - 5
DP  - 1992 Nov
TI  - Cloning of an ovine 11 beta-hydroxysteroid dehydrogenase complementary 
      deoxyribonucleic acid: tissue and temporal distribution of its messenger 
      ribonucleic acid during fetal and neonatal development.
PG  - 2120-6
AB  - Glucocorticoids promote the development of many organ systems vital for 
      extrauterine survival, and fetal cortisol provides the trigger for birth in 
      sheep. The activity of glucocorticoids may be influenced at a cellular level by 
      11 beta-hydroxysteroid dehydrogenase (11 beta-HSD), which is responsible for the 
      interconversion of cortisol and cortisone. To examine 11 beta-HSD gene expression 
      during fetal development, two overlapping clones which yield a 1.4 kilobase (kb) 
      complementary DNA encoding sheep 11 beta-HSD from a liver library were isolated 
      by using a rat 11 beta-HSD cDNA as the probe. This cDNA contains a 879 base pair 
      open reading frame for a protein of 292 amino acids that has more than 70% 
      sequence identity to rat and human 11 beta-HSDs. To define the tissue 
      distribution of 11 beta-HSD messenger RNA in sheep, selected tissues were 
      collected from one fetus at day 130 and term (approximately 145 days), and from a 
      nonpregnant ewe. Cellular RNA was extracted and subjected to Northern blot 
      analysis, and a single 1.8 kb transcript was detected in the fetal and adult 
      liver, lung, hypothalamus, anterior pituitary, and placenta. This was 
      undetectable in adrenals and kidneys, but a smaller (1.5 kb) transcript was 
      present in fetal and adult kidney RNA. The relative abundance of 11 beta-HSD mRNA 
      was greatest in fetal and adult livers, and it was much higher in adult liver, 
      lung, and kidney than in the corresponding fetal tissues. To examine whether 11 
      beta-HSD gene expression is developmentally regulated in the fetal sheep, liver, 
      lung, and kidney tissues were taken from fetuses at day 60-70, day 100-110, day 
      125-130, at term, and from newborn lambs (24-48 h old). In the lung and kidney, 
      the relative abundance of 11 beta-HSD mRNA did not change from day 60 to term but 
      increased in the lungs of newborn lambs. In contrast, 11 beta-HSD mRNA levels in 
      the liver increased between day 125 and term and rose further in the newborn. 
      Collectively, these results demonstrate that 11 beta-HSD gene expression in sheep 
      is regulated in a tissue-specific and developmentally programmed manner.
FAU - Yang, K
AU  - Yang K
AD  - Lawson Research Institute, St. Joseph's Health Centre, London, Ontario, Canada.
FAU - Smith, C L
AU  - Smith CL
FAU - Dales, D
AU  - Dales D
FAU - Hammond, G L
AU  - Hammond GL
FAU - Challis, J R
AU  - Challis JR
LA  - eng
SI  - GENBANK/D13201
SI  - GENBANK/D13202
SI  - GENBANK/D13203
SI  - GENBANK/M94783
SI  - GENBANK/M94784
SI  - GENBANK/M94785
SI  - GENBANK/S46205
SI  - GENBANK/X64299
SI  - GENBANK/X64300
SI  - GENBANK/X69561
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (RNA, Messenger)
RN  - 9007-49-2 (DNA)
RN  - EC 1.1.- (Hydroxysteroid Dehydrogenases)
RN  - EC 1.1.1.146 (11-beta-Hydroxysteroid Dehydrogenases)
SB  - IM
GS  - 11&bgr;-HSD
MH  - 11-beta-Hydroxysteroid Dehydrogenases
MH  - Adrenal Glands/chemistry/embryology/enzymology
MH  - Animals
MH  - Animals, Newborn/*genetics
MH  - Base Sequence
MH  - Blotting, Northern
MH  - Cloning, Molecular
MH  - DNA/analysis/*genetics
MH  - Embryonic and Fetal Development/*genetics
MH  - Female
MH  - Fetus/chemistry
MH  - Gene Expression
MH  - Hydroxysteroid Dehydrogenases/*genetics
MH  - Hypothalamus/chemistry/embryology/enzymology
MH  - Liver/*chemistry/embryology/enzymology
MH  - Lung/chemistry/embryology/enzymology
MH  - Molecular Sequence Data
MH  - Pituitary Gland, Anterior/chemistry/embryology/enzymology
MH  - Placenta/chemistry/embryology/enzymology
MH  - Pregnancy
MH  - RNA, Messenger/*analysis/genetics
MH  - Sheep
MH  - Tissue Distribution
EDAT- 1992/11/01 00:00
MHDA- 1992/11/01 00:01
CRDT- 1992/11/01 00:00
PHST- 1992/11/01 00:00 [pubmed]
PHST- 1992/11/01 00:01 [medline]
PHST- 1992/11/01 00:00 [entrez]
AID - 10.1210/endo.131.5.1425412 [doi]
PST - ppublish
SO  - Endocrinology. 1992 Nov;131(5):2120-6. doi: 10.1210/endo.131.5.1425412.