PMID- 1425770
OWN - NLM
STAT- MEDLINE
DCOM- 19921223
LR  - 20061115
IS  - 0171-9335 (Print)
IS  - 0171-9335 (Linking)
VI  - 58
IP  - 2
DP  - 1992 Aug
TI  - Macromolecular structure of reassembled neurofilaments as revealed by the 
      quick-freeze deep-etch mica method: difference between NF-M and NF-H subunits in 
      their ability to form cross-bridges.
PG  - 331-45
AB  - Neurofilament (NF) structure and ability to form cross-bridges were examined by 
      quick-freeze deep-etch mica and low-angle rotary-shadow electron microscopy in 
      NFs purified from bovine spinal cord and reassembled in various combinations of 
      NF subunits. When NFs were reassembled from triplet proteins, NF-L, NF-M and 
      NF-H, they were oriented randomly and often fragmented, but their elongated 
      filaments (12-15 nm wide) and the cross-bridges (4-5 nm wide) connecting them 
      were similar in appearance to those of isolated bovine NFs or in vivo rat NFs. 
      Projections extended from the wall of the core filament in almost the same 
      pattern as the cross-bridges and were the same in width and interval (minimum 
      interval, 20-25 nm) as the cross-bridges. Projections were more conspicuous when 
      core filaments were separated by 60 to 80 nm or more, while cross-bridges were 
      more conspicuous when core filaments were close to each other. Projections or 
      cross-bridges extended bilaterally at intervals of 20 to 25 nm where core 
      filaments expanded and formed a network between filaments which were far from one 
      another. When NFs were reconstructed from NF-L alone, only core filaments 
      appeared, the same width as the filaments of triplet NFs. The core filaments were 
      occasionally in almost direct contact with each other, with no projection or 
      cross-bridge. When NFs were reassembled from NF-M alone or NF-L + NF-M, although 
      NF-M core filaments were shorter and slightly thinner than NF-L + NF-M core 
      filaments, both had projections, and both had cross-bridges, but cross-bridges 
      were less evident. Cross-bridges were almost the same in width as those of 
      triplet NFs, but significantly shorter and much less frequent although the 
      minimum interval was the same, and core filaments were not attached to each 
      other. In contrast, when NFs were reconstituted from NF-H alone or NF-L + NF-H, 
      both had conspicuous projections and cross-bridges, similar to those of triplet 
      NFs. Thus, when NFs contained NF-H, they formed frequent cross-bridges and long 
      projections with extensive peripheral branching. When NFs contained NF-M but no 
      NF-H, they tended to form cross-bridges, and to form projections that were 
      shorter and straighter and without peripheral branching. That is, there appears 
      to be a significant difference between NF-M and NF-H in ability to form 
      cross-bridges and thus in interaction with adjacent NFs.
FAU - Gotow, T
AU  - Gotow T
AD  - Department of Anatomy, Osaka University Medical School, Japan.
FAU - Takeda, M
AU  - Takeda M
FAU - Tanaka, T
AU  - Tanaka T
FAU - Hashimoto, P H
AU  - Hashimoto PH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Eur J Cell Biol
JT  - European journal of cell biology
JID - 7906240
RN  - 0 (Neurofilament Proteins)
RN  - 108688-71-7 (neurofilament protein H)
RN  - 111365-29-8 (neurofilament protein M)
SB  - IM
MH  - Animals
MH  - Brain Chemistry
MH  - Cattle
MH  - Freeze Etching
MH  - Intermediate Filaments/chemistry/*ultrastructure
MH  - Neurofilament Proteins/chemistry/*ultrastructure
MH  - Rats
MH  - Rats, Wistar
MH  - Spinal Cord/chemistry
EDAT- 1992/08/01 00:00
MHDA- 1992/08/01 00:01
CRDT- 1992/08/01 00:00
PHST- 1992/08/01 00:00 [pubmed]
PHST- 1992/08/01 00:01 [medline]
PHST- 1992/08/01 00:00 [entrez]
PST - ppublish
SO  - Eur J Cell Biol. 1992 Aug;58(2):331-45.