PMID- 1427905
OWN - NLM
STAT- MEDLINE
DCOM- 19921217
LR  - 20190902
IS  - 0888-7543 (Print)
IS  - 0888-7543 (Linking)
VI  - 14
IP  - 3
DP  - 1992 Nov
TI  - Construction and characterization of a region-specific microdissection library 
      from human chromosome 2q35-q37.
PG  - 769-74
AB  - A region-specific genomic library for human chromosome 2q35-q37 has been 
      constructed using the microdissection and polymerase chain reaction-mediated 
      linker-adaptor microcloning method. Twenty fragments from the chromosome region 
      2q35-q37 were dissected and a library consisting of 20,000 recombinant 
      microclones was obtained. The insert size ranged between 50 and 800 bp, with a 
      mean of approximately 270 bp. About 50-60% of the microclones contained unique 
      sequences. The microdissection library has been demonstrated to derive from the 
      dissected region 2q35-q37 by chromosome painting using the fluorescence in situ 
      hybridization (FISH) technique. Southern blot analysis of the unique sequence 
      microclones from the library showed that 54% (26/48) of the clones are of human 
      origin and chromosome 2 specific. Four of these microclones have been further 
      mapped to the 2q37 region by using a cell hybrid containing only 2q37. The unique 
      sequence microclones have also been characterized for their insert size and the 
      hybridizing genomic fragments cleaved with HindIII. As shown previously, these 
      microclones will be useful in isolating corresponding yeast artificial chromosome 
      (YAC) clones with large inserts for high-resolution physical mapping and also in 
      screening cDNA libraries to isolate expressed gene sequences as candidate genes 
      to facilitate search for the crucial genes underlying genetic diseases and 
      specific forms of cancer assigned to the region.
FAU - Yu, J
AU  - Yu J
AD  - Eleanor Roosevelt Institute for Cancer Research, University of Colorado Health 
      Sciences Center, Denver 80206.
FAU - Tong, S
AU  - Tong S
FAU - Yang-Feng, T
AU  - Yang-Feng T
FAU - Kao, F T
AU  - Kao FT
LA  - eng
GR  - HD-17749/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Genomics
JT  - Genomics
JID - 8800135
SB  - IM
MH  - Blotting, Southern
MH  - Cell Line
MH  - *Chromosomes, Human, Pair 2
MH  - Cloning, Molecular
MH  - *Genomic Library
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Polymerase Chain Reaction
MH  - Repetitive Sequences, Nucleic Acid
EDAT- 1992/11/01 00:00
MHDA- 1992/11/01 00:01
CRDT- 1992/11/01 00:00
PHST- 1992/11/01 00:00 [pubmed]
PHST- 1992/11/01 00:01 [medline]
PHST- 1992/11/01 00:00 [entrez]
AID - S0888-7543(05)80183-1 [pii]
AID - 10.1016/s0888-7543(05)80183-1 [doi]
PST - ppublish
SO  - Genomics. 1992 Nov;14(3):769-74. doi: 10.1016/s0888-7543(05)80183-1.