PMID- 1427906 OWN - NLM STAT- MEDLINE DCOM- 19921217 LR - 20220316 IS - 0888-7543 (Print) IS - 0888-7543 (Linking) VI - 14 IP - 3 DP - 1992 Nov TI - Subregional mapping of 13 single-copy genes on the long arm of chromosome 12 by fluorescence in situ hybridization. PG - 775-9 AB - Subregional localization of 13 single-copy DNA sequences previously assigned to the long arm of chromosome 12 has been performed using the fluorescence in situ hybridization (FISH) technique. The following order is suggested for the 13 mapped genes: cen-->COL2A1-->(VDR-D12S15)-->(D12S17-D12S4++ +-D12S14-D12S6)-->D12S8-->(IAPP-MGF- D12S7-D12S12)-->IGF1-->qter. Eight of the mapped genes clustered at two regions, one at 12q13 (D12S17-D12S4-D12S14-D12S6) and the other at 12q22 (IAPP-MGF-D12S7-D12S12). Our results show that single-copy DNA sequences as small as 500 bp can be successfully mapped by FISH. FAU - Mathew, S AU - Mathew S AD - Laboratory of Cancer Genetics, Memorial Sloan-Kettering Cancer Center, New York, New York 10021. FAU - Murty, V V AU - Murty VV FAU - Hunziker, W AU - Hunziker W FAU - Chaganti, R S AU - Chaganti RS LA - eng GR - CA-05826/CA/NCI NIH HHS/United States GR - CA-34775/CA/NCI NIH HHS/United States PT - Journal Article PT - Research Support, U.S. Gov't, P.H.S. PL - United States TA - Genomics JT - Genomics JID - 8800135 SB - IM MH - Chromosome Mapping MH - *Chromosomes, Human, Pair 12 MH - Humans MH - In Situ Hybridization, Fluorescence/methods EDAT- 1992/11/01 00:00 MHDA- 1992/11/01 00:01 CRDT- 1992/11/01 00:00 PHST- 1992/11/01 00:00 [pubmed] PHST- 1992/11/01 00:01 [medline] PHST- 1992/11/01 00:00 [entrez] AID - S0888-7543(05)80184-3 [pii] AID - 10.1016/s0888-7543(05)80184-3 [doi] PST - ppublish SO - Genomics. 1992 Nov;14(3):775-9. doi: 10.1016/s0888-7543(05)80184-3.