PMID- 1431552
OWN - NLM
STAT- MEDLINE
DCOM- 19921127
LR  - 20190516
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 51
IP  - 2
DP  - 1992 Feb
TI  - The up- and down-modulation of immunoglobulin G Fc receptors and complement 
      receptors on activated human neutrophils depends on the nature of activator.
PG  - 157-63
AB  - Human neutrophils were activated with soluble stimuli, 
      formyl-methionyl-leucyl-phenylalanine (fMLP) or ionophore A23187, and with 
      opsonized particles, zymosan or Streptococcus pneumoniae bacteria. Monoclonal 
      antibodies and flow cytometry were used to assess the expression of Fc-gamma 
      receptors (FcRI, FcRII, FcRIII) and complement receptors (CR1, CR3). The role of 
      extracellular calcium and magnesium in the modulation of receptor expression was 
      also examined. The low-level expression of FcRI was not affected by any activator 
      tested. fMLP and A23187 did not alter the expression of FcRII, whereas a 
      significant, Ca(2+)- and Mg(2+)-independent down-modulation was observed upon 
      activation with opsonized particles. All activators clearly decreased the surface 
      expression of FcRIII in the presence of Ca2+ and Mg2+, probably as a consequence 
      of shedding of the phosphatidylinositol-glycan-anchored receptor protein. The 
      removal of calcium and magnesium blocked the shedding of FcRIII caused by soluble 
      stimuli, whereas it retarded but did not abolish the fall in FcRIII expression 
      when cells were incubated with opsonized particles. This fall was likely due to 
      internalization of the receptor molecules while the shedding was blocked. A rapid 
      increase in CR1 and CR3 expression was seen upon activation with soluble stimuli. 
      The change in CR1 expression was independent of extracellular Ca2+ and Mg2+. The 
      increase in CR3 number required an influx of divalent cations. No total 
      up-modulation of complement receptors occurred when neutrophils were activated 
      with opsonized particles. However, the kinetic analysis revealed a temporary 
      up-modulation that was followed by a down-modulation. The results indicate that 
      the expression of both Fc-gamma and complement receptors on human neutrophils is 
      changed upon activation and that the up- and down-modulation of these receptors 
      depends on the nature of activator. We also suggest that in neutrophils the 
      FcRIII down-modulation is the result of both receptor shedding and 
      internalization, while FcRII is down-modulated by receptor internalization.
FAU - Leino, L
AU  - Leino L
AD  - Department of Hematology, Turku University Central Hospital, Finland.
FAU - Lilius, E M
AU  - Lilius EM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (Opsonin Proteins)
RN  - 0 (Receptors, Complement)
RN  - 0 (Receptors, IgG)
RN  - 37H9VM9WZL (Calcimycin)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - I38ZP9992A (Magnesium)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Calcimycin/pharmacology
MH  - Calcium/physiology
MH  - Down-Regulation
MH  - Endocytosis
MH  - Extracellular Space/metabolism
MH  - Humans
MH  - In Vitro Techniques
MH  - Magnesium/physiology
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - Neutrophils/*metabolism
MH  - Opsonin Proteins
MH  - Receptors, Complement/*metabolism
MH  - Receptors, IgG/*metabolism
MH  - Up-Regulation
EDAT- 1992/02/01 00:00
MHDA- 1992/02/01 00:01
CRDT- 1992/02/01 00:00
PHST- 1992/02/01 00:00 [pubmed]
PHST- 1992/02/01 00:01 [medline]
PHST- 1992/02/01 00:00 [entrez]
AID - 10.1002/jlb.51.2.157 [doi]
PST - ppublish
SO  - J Leukoc Biol. 1992 Feb;51(2):157-63. doi: 10.1002/jlb.51.2.157.