PMID- 1434599
OWN - NLM
STAT- MEDLINE
DCOM- 19921204
LR  - 20190817
IS  - 0022-4804 (Print)
IS  - 0022-4804 (Linking)
VI  - 53
IP  - 5
DP  - 1992 Nov
TI  - Limiting oxygen delivery attenuates intestinal reperfusion injury.
PG  - 485-9
AB  - Since free radical-mediated injury is dependent on the reintroduction of oxygen 
      into ischemic tissues, restriction of oxygen content in the initial reperfusate 
      has therapeutic potential. The degree to which oxygen must be restricted is 
      crucial since hypoxic injury would continue if reperfusion O2 delivery remained 
      below the ischemic threshold of the tissue. We examined this treatment strategy 
      in 20 pump-perfused intestinal preparations subjected to 30 min of flow 
      interruption. The oxygen content of the reperfusate was varied by utilizing 
      arterial (A) or venous (V) blood; as a further modification, we also performed 
      experiments in which hemodiluted arterial blood (HD) was the reperfusate at 
      normal (NHD) and high (HHD) flow rates. The flow rates and O2 contents of the 
      reperfusates were adjusted to produce either high (approximately 12 ml O2/min/100 
      g) or low (approximately 8 ml O2/min/100 g) levels of O2 delivery. Histologic 
      sections, obtained after ischemia and after 1 hr of reperfusion, were blindly 
      evaluated for mucosal injury (1 = normal to 5 = severe injury). Immediately after 
      30 min of ischemia, all groups had comparable histologic grades (A 2.0 +/- 0.3, V 
      1.8 +/- 0.3, NHD 1.6 +/- 0.3, HHD 2.3 +/- 0.3). One hour after reperfusion, 
      intestines reperfused with blood with high O2 content and hence high O2 delivery 
      showed significantly more damage (P < 0.001) than those with exposed to low O2 
      delivery during reperfusion: A 3.9 +/- 0.5 and HHD 4.4 +/- 0.4 versus V 2.7 +/- 
      0.5 and NHD 2.9 +/- 0.3.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Clark, E T
AU  - Clark ET
AD  - Department of Surgery, University of Chicago, Illinois 60637.
FAU - Gewertz, B L
AU  - Gewertz BL
LA  - eng
GR  - T32 HL07665-02/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Surg Res
JT  - The Journal of surgical research
JID - 0376340
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Animals
MH  - Arteries
MH  - Intestines/*blood supply/pathology
MH  - Ischemia
MH  - Male
MH  - Necrosis
MH  - Oxygen/*administration & dosage/blood
MH  - Rats
MH  - Rats, Wistar
MH  - Reperfusion Injury/*prevention & control
MH  - Veins
EDAT- 1992/11/01 00:00
MHDA- 1992/11/01 00:01
CRDT- 1992/11/01 00:00
PHST- 1992/11/01 00:00 [pubmed]
PHST- 1992/11/01 00:01 [medline]
PHST- 1992/11/01 00:00 [entrez]
AID - 0022-4804(92)90094-G [pii]
AID - 10.1016/0022-4804(92)90094-g [doi]
PST - ppublish
SO  - J Surg Res. 1992 Nov;53(5):485-9. doi: 10.1016/0022-4804(92)90094-g.