PMID- 1443123
OWN - NLM
STAT- MEDLINE
DCOM- 19921223
LR  - 20171213
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 263
IP  - 5 Pt 1
DP  - 1992 Nov
TI  - Inhibition of hepatic ketogenesis by tumor necrosis factor-alpha in rats.
PG  - E897-902
AB  - Tumor necrosis factor-alpha (TNF-alpha) stimulates hepatic lipogenesis. 
      Therefore, it could play a role in the control of ketogenesis. To test this 
      hypothesis, we measured simultaneously free fatty acids (FFA; [1-13C]palmitate) 
      and ketone body (KB; [3,4-13C2]acetoacetate) kinetics, before and after 
      intraperitoneal injection of saline or TNF-alpha, in postabsorptive rats or rats 
      starved for 24 h. In both groups of rats, TNF-alpha injection did not modify 
      insulinemia and induced a moderate increase of FFA concentrations and appearance 
      rates (P < 0.05). Despite increased FFA availability, ketogenesis was impaired 
      after TNF-alpha injection, as shown by lower KB concentrations and appearance 
      rates; this effect was more important in postabsorptive than in starved rats. The 
      percentage of FFA flux used for ketogenesis was decreased by TNF-alpha in the 
      postabsorptive group (P < 0.05) and starved (P < 0.05) rats. In both groups, 
      maximal liver acetyl-coenzyme A carboxylase activity and estimated 
      phosphorylation state were not modified by TNF-alpha injection, but hepatic 
      concentrations of citrate were increased (P < 0.05). This increased citrate level 
      could be related to a mobilization of glucose stored as glycogen since liver 
      glycogen was decreased by TNF-alpha injection (P < 0.05). In conclusion, 
      TNF-alpha injection in rats decreased hepatic ketogenesis. This action could be 
      related to an increased mobilization and utilization of carbohydrate stores.
FAU - Beylot, M
AU  - Beylot M
AD  - Institut National de la Sante et de la Recherche Medicale U.197 Faculte A. 
      Carrel, Lyon, France.
FAU - Vidal, H
AU  - Vidal H
FAU - Mithieux, G
AU  - Mithieux G
FAU - Odeon, M
AU  - Odeon M
FAU - Martin, C
AU  - Martin C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 0 (Fatty Acids, Nonesterified)
RN  - 0 (Ketone Bodies)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 9005-79-2 (Glycogen)
SB  - IM
MH  - Absorption
MH  - Animals
MH  - Fatty Acids, Nonesterified/metabolism
MH  - Glycogen/metabolism
MH  - Ketone Bodies/antagonists & inhibitors/*biosynthesis
MH  - Kinetics
MH  - Liver/*metabolism
MH  - Male
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Starvation/metabolism
MH  - Tumor Necrosis Factor-alpha/*pharmacology
EDAT- 1992/11/01 00:00
MHDA- 1992/11/01 00:01
CRDT- 1992/11/01 00:00
PHST- 1992/11/01 00:00 [pubmed]
PHST- 1992/11/01 00:01 [medline]
PHST- 1992/11/01 00:00 [entrez]
AID - 10.1152/ajpendo.1992.263.5.E897 [doi]
PST - ppublish
SO  - Am J Physiol. 1992 Nov;263(5 Pt 1):E897-902. doi: 
      10.1152/ajpendo.1992.263.5.E897.