PMID- 1443226
OWN - NLM
STAT- MEDLINE
DCOM- 19921222
LR  - 20181130
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 263
IP  - 5 Pt 2
DP  - 1992 Nov
TI  - Growth hormone-releasing hormone antibodies suppress sleep and prevent 
      enhancement of sleep after sleep deprivation.
PG  - R1078-85
AB  - Previous reports suggest that the hypothalamic growth hormone-releasing hormone 
      (GHRH) promotes sleep, especially non-rapid-eye-movement sleep (NREMS). To 
      evaluate the role of endogenous GHRH in sleep regulation, the effects of 
      antibodies to rat GHRH (GHRH-ab) were studied on normal sleep, brain temperature 
      (Tbr), and GH secretion in experiment I and on enhanced sleep after sleep 
      deprivation in experiment II. In experiment I, affinity-purified GHRH-ab (50 and 
      200 micrograms) raised in goats and a control goat immunoglobulin G (IgG) 
      preparation were injected intracerebroventricularly (icv) in rats 1 h before the 
      onset of the light cycle, and sleep-wake activity and Tbr were recorded for the 
      next 12 or 23 h. Both doses of GHRH-ab suppressed NREMS and REMS throughout the 
      light cycle. Sleep durations at night were normal. Electroencephalographic (EEG) 
      slow-wave activity, characterized by EEG slow-wave amplitudes, was reduced after 
      GHRH-ab during both the light and the dark cycles. Plasma GH concentrations 
      measured 6-12 h after injection of GHRH-ab (200 micrograms) were diminished. Both 
      the control IgG and GHRH-ab elicited fever. In experiment II, the sleep-wake 
      activity and Tbr of rats were recorded for 24 h in three experimental conditions: 
      base-line with icv injection of IgG, 3-h sleep deprivation with icv IgG 
      injection, and 3-h sleep deprivation with icv GHRH-ab (200 micrograms). After 
      sleep deprivation (+IgG), a prompt increase in EEG slow-wave activity (power 
      density analysis) and late increases in NREMS and REMS durations were 
      found.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Obal, F Jr
AU  - Obal F Jr
AD  - Department of Physiology and Biophysics, University of Tennessee, Memphis 38163.
FAU - Payne, L
AU  - Payne L
FAU - Opp, M
AU  - Opp M
FAU - Alfoldi, P
AU  - Alfoldi P
FAU - Kapas, L
AU  - Kapas L
FAU - Krueger, J M
AU  - Krueger JM
LA  - eng
GR  - NS-27250/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 0 (Antibodies)
RN  - 9002-72-6 (Growth Hormone)
RN  - 9034-39-3 (Growth Hormone-Releasing Hormone)
SB  - IM
MH  - Animals
MH  - Antibodies/administration & dosage/*immunology
MH  - Body Temperature
MH  - Brain/physiology
MH  - Growth Hormone/metabolism
MH  - Growth Hormone-Releasing Hormone/*immunology
MH  - Injections, Intraventricular
MH  - Male
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sleep/*physiology
MH  - Sleep Deprivation/*physiology
EDAT- 1992/11/11 19:15
MHDA- 1992/11/11 19:16
CRDT- 1992/11/11 19:15
PHST- 1992/11/11 19:15 [pubmed]
PHST- 1992/11/11 19:16 [medline]
PHST- 1992/11/11 19:15 [entrez]
AID - 10.1152/ajpregu.1992.263.5.R1078 [doi]
PST - ppublish
SO  - Am J Physiol. 1992 Nov;263(5 Pt 2):R1078-85. doi: 
      10.1152/ajpregu.1992.263.5.R1078.