PMID- 1445491
OWN - NLM
STAT- MEDLINE
DCOM- 19921214
LR  - 20190718
IS  - 0021-9150 (Print)
IS  - 0021-9150 (Linking)
VI  - 97
IP  - 1
DP  - 1992 Nov
TI  - Transforming growth factor-beta 1 and interleukin-1 beta stimulate LDL receptor 
      activity in Hep G2 cells.
PG  - 21-8
AB  - The effect of transforming growth factor-beta 1 (TGF-beta 1) and interleukin-1 
      beta (IL-1 beta) on LDL receptor in Hep G2 cells was investigated. A greater than 
      two-fold stimulation of the binding and internalisation of [125I]-labelled LDL at 
      37 degrees C was observed after an 18-h incubation of the cells with TGF-beta 1 
      at 50 ng/ml and IL-1 beta at 11,700 units/ml compared with control cells. 
      Scatchard analysis of the binding of [125I]-labelled LDL at 4 degrees C after an 
      18-h incubation of the cells with 1170 units/ml IL-1 beta and 5 ng/ml TGF-beta 1 
      showed that they were both acting primarily by increasing LDL receptor number. 
      The increase in LDL receptor activity could not be attributed to an increase in 
      cell proliferation as TGF-beta 1 at concentrations from 0.05 ng/ml to 50 ng/ml 
      had no significant effect on either cell number or [3H]thymidine incorporation 
      into DNA whilst IL-1 beta inhibited DNA synthesis by more than 80% at a 
      concentration of 11,700 units/ml but had significant effect on cell number. 
      Cholesterol biosynthesis from [14C]acetate, in contrast to the stimulation of LDL 
      receptor activity, was inhibited by approximately two-fold by incubation with 
      TGF-beta 1 at 50 ng/ml and IL-1 beta at 11,700 units/ml.
FAU - Moorby, C D
AU  - Moorby CD
AD  - Department of Pathology, University of Cambridge, UK.
FAU - Gherardi, E
AU  - Gherardi E
FAU - Dovey, L
AU  - Dovey L
FAU - Godliman, C
AU  - Godliman C
FAU - Bowyer, D E
AU  - Bowyer DE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Atherosclerosis
JT  - Atherosclerosis
JID - 0242543
RN  - 0 (Interleukin-1)
RN  - 0 (Receptors, LDL)
RN  - 0 (Transforming Growth Factor beta)
RN  - 9007-49-2 (DNA)
RN  - 97C5T2UQ7J (Cholesterol)
SB  - IM
MH  - Animals
MH  - Cholesterol/biosynthesis
MH  - DNA/biosynthesis
MH  - Dose-Response Relationship, Drug
MH  - Humans
MH  - Interleukin-1/*pharmacology
MH  - Liver Neoplasms, Experimental/*metabolism
MH  - Receptors, LDL/*metabolism
MH  - Transforming Growth Factor beta/*pharmacology
MH  - Tumor Cells, Cultured/metabolism
EDAT- 1992/11/01 00:00
MHDA- 1992/11/01 00:01
CRDT- 1992/11/01 00:00
PHST- 1992/11/01 00:00 [pubmed]
PHST- 1992/11/01 00:01 [medline]
PHST- 1992/11/01 00:00 [entrez]
AID - 0021-9150(92)90047-K [pii]
AID - 10.1016/0021-9150(92)90047-k [doi]
PST - ppublish
SO  - Atherosclerosis. 1992 Nov;97(1):21-8. doi: 10.1016/0021-9150(92)90047-k.