PMID- 1445890
OWN - NLM
STAT- MEDLINE
DCOM- 19921230
LR  - 20190613
IS  - 0006-2960 (Print)
IS  - 0006-2960 (Linking)
VI  - 31
IP  - 46
DP  - 1992 Nov 24
TI  - Direct measurement of the weak interactions between a mouse Fc receptor (Fc gamma 
      RII) and IgG1 in the absence and presence of hapten: a total internal reflection 
      fluorescence microscopy study.
PG  - 11562-6
AB  - Total internal reflection fluorescence microscopy (TIRFM) has been used to 
      directly measure the weak dissociation constants of IgG with a mouse IgG receptor 
      (moFc gamma RII) that has been purified and reconstituted into 
      substrate-supported planar membranes. Dissociation constants were measured for 
      three different mouse monoclonal anti-dinitrophenyl (DNP) IgG1 antibodies and for 
      polyclonal mouse IgG, in the absence and presence of saturating amounts of hapten 
      (DNP-glycine). The dissociation constant for polyclonal mouse IgG was 3 microM, 
      which agrees well with previous results. The dissociation constants for the three 
      monoclonal antibodies with moFc gamma RII ranged from 2 microM to 3 microM and 
      were not statistically different, suggesting that changes in moFc gamma RII 
      dissociation constants which may exist within the IgG1 subclass are less than the 
      error of the TIRFM measurements (approximately 20%). The measured IgG1-moFc gamma 
      RII dissociation constants were not different for individual monoclonal 
      antibodies in the absence or presence of saturating concentrations of 
      DNP-glycine, directly showing that possible allosteric changes which might occur 
      upon hapten binding and affect the equilibrium characteristics of Fc receptor 
      binding are small. This work demonstrates a new approach for quantitatively 
      examining the effects of solution components on weak receptor-ligand 
      interactions.
FAU - Hsieh, H V
AU  - Hsieh HV
AD  - Department of Chemistry, University of North Carolina, Chapel Hill 27599-3290.
FAU - Poglitsch, C L
AU  - Poglitsch CL
FAU - Thompson, N L
AU  - Thompson NL
LA  - eng
GR  - GM37145/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Biochemistry
JT  - Biochemistry
JID - 0370623
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Haptens)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Receptors, Fc)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal
MH  - Cell Line
MH  - *Haptens
MH  - Immunoglobulin G/*metabolism
MH  - Mice
MH  - Microscopy, Fluorescence
MH  - Receptors, Fc/*metabolism
MH  - Substrate Specificity
EDAT- 1992/11/24 00:00
MHDA- 1992/11/24 00:01
CRDT- 1992/11/24 00:00
PHST- 1992/11/24 00:00 [pubmed]
PHST- 1992/11/24 00:01 [medline]
PHST- 1992/11/24 00:00 [entrez]
AID - 10.1021/bi00161a039 [doi]
PST - ppublish
SO  - Biochemistry. 1992 Nov 24;31(46):11562-6. doi: 10.1021/bi00161a039.