PMID- 1446622
OWN - NLM
STAT- MEDLINE
DCOM- 19921230
LR  - 20181130
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 131
IP  - 6
DP  - 1992 Dec
TI  - Leydig cell peroxisomes and sterol carrier protein-2 in luteinizing 
      hormone-deprived rats.
PG  - 2839-45
AB  - We investigated the effects of 8 days of LH withdrawal on rat Leydig cell 
      peroxisomal volume, total and intraperoxisomal catalase and sterol carrier 
      protein-2 (SCP2) contents, and LH-stimulated testosterone secretion in vitro. 
      Three groups of adult male Sprague-Dawley rats, i.e. control, TE-implanted 
      (testosterone-17 beta-estradiol-filled Silastic implants to suppress LH), and 
      TELH-implanted (TE-implanted and LH replacement via Alzet mini osmotic pumps), 
      were used. After 8 days, Leydig cell organelle volumes (stereology), 
      intraperoxisomal catalase and SCP2 contents (immunocytochemistry), LH-stimulated 
      testosterone secretion by isolated Leydig cells in vitro (determined by RIA), and 
      total catalase and SCP2 contents in equal numbers of Leydig cells (immunoblot 
      analyses) were determined. Results showed that the TELH-implanted rats were 
      identical to controls in every parameter tested. Testis volume and Leydig cell 
      number per testis in control and TE-implanted rats were not significantly 
      different; however, reductions (P < 0.05) were observed in the average volume of 
      a Leydig cell (one third of controls) and the volume of Leydig cells per testis. 
      All Leydig cell organelle volumes tested were significantly lower in TE-implanted 
      rats than in the controls; however, the volumes of smooth endoplasmic reticulum 
      (SER) and peroxisomes were the most reduced (lowered to one sixth of control 
      values). LH-stimulated testosterone secretion per Leydig cell in vitro correlated 
      well with these changes in the volumes of Leydig cell SER and peroxisomes. 
      Intraperoxisomal catalase in Leydig cells was unchanged in TE-implanted rats, 
      although immunoblotting demonstrated a loss of total catalase content (which 
      reflected the reduction in the volume of peroxisomes). SCP2 in Leydig cells of 
      TE-implanted rats was undetectable with immunoblot analysis (explained by the 
      reductions in Leydig cell peroxisome volume and intraperoxisomal SCP2). These 
      results demonstrate that the organelles SER and peroxisomes and the protein SCP2 
      in Leydig cells are more LH dependent than the other organelles (e.g. 
      mitochondria, lysosomes) and protein catalase, respectively. Moreover, the 
      findings of this study are consistent with the hypothesis that Leydig cell 
      peroxisomes play a significant role in testosterone production.
FAU - Mendis-Handagama, S M
AU  - Mendis-Handagama SM
AD  - Department of Population Dynamics, Johns Hopkins School of Hygiene and Public 
      Health, Kennedy Institute, Baltimore, Maryland 21205.
FAU - Watkins, P A
AU  - Watkins PA
FAU - Gelber, S J
AU  - Gelber SJ
FAU - Scallen, T J
AU  - Scallen TJ
LA  - eng
GR  - F32-DK-08312-02/DK/NIDDK NIH HHS/United States
GR  - HD-07204/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (Carrier Proteins)
RN  - 0 (Plant Proteins)
RN  - 0 (Sterols)
RN  - 0 (sterol carrier proteins)
RN  - 3XMK78S47O (Testosterone)
RN  - 4TI98Z838E (Estradiol)
RN  - 9002-67-9 (Luteinizing Hormone)
RN  - EC 1.11.1.6 (Catalase)
SB  - IM
MH  - Animals
MH  - Carrier Proteins/*metabolism
MH  - Catalase/metabolism
MH  - Estradiol/pharmacology
MH  - Immunoblotting
MH  - Leydig Cells/drug effects/*metabolism/*ultrastructure
MH  - Luteinizing Hormone/*administration & dosage/pharmacology
MH  - Male
MH  - Microbodies/enzymology/*ultrastructure
MH  - Organelles/drug effects/ultrastructure
MH  - *Plant Proteins
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sterols/*metabolism
MH  - Testis/anatomy & histology/drug effects
MH  - Testosterone/metabolism/pharmacology
EDAT- 1992/12/01 00:00
MHDA- 1992/12/01 00:01
CRDT- 1992/12/01 00:00
PHST- 1992/12/01 00:00 [pubmed]
PHST- 1992/12/01 00:01 [medline]
PHST- 1992/12/01 00:00 [entrez]
AID - 10.1210/endo.131.6.1446622 [doi]
PST - ppublish
SO  - Endocrinology. 1992 Dec;131(6):2839-45. doi: 10.1210/endo.131.6.1446622.