PMID- 1448442
OWN - NLM
STAT- MEDLINE
DCOM- 19921224
LR  - 20190902
IS  - 0901-9928 (Print)
IS  - 0901-9928 (Linking)
VI  - 71
IP  - 5
DP  - 1992 Nov
TI  - Excessive accumulation of hepatic copper in LEC rats aged 80 days without 
      hepatitis and 130 days with hepatitis.
PG  - 321-4
AB  - The Cu concentration was about 40 and 60 times higher in the liver in Long-Evans 
      with a cinnamon-like coat color (LEC) rats aged 80 days (without hepatitis) and 
      130 days (with hepatitis), respectively than in the liver in Fischer rats. Most 
      hepatic Cu was recovered in the cytosol fraction. Furthermore, about 96% and 84% 
      of the cytosolic Cu was found in the metallothionein region on a Sephadex G-75 
      column in LEC rats aged 80 and 130 days, respectively. The hepatic 
      metallothionein concentration was about 130 to 140 times higher in LEC rats than 
      in Fischer rats when the concentration was expressed as metallothionein-bound Cu. 
      Three forms of Cu-metallothionein were isolated by DEAE-cartridge. Although the 
      concentration of hepatic Cu-metallothionein and its composition of polymorphic 
      form were not changed greatly in hepatitis phase (in the 130-day-old LEC rats), 
      activities of serum enzymes, aspartate aminotransferase (GOT) and alanine 
      aminotransferase (GPT) were increased significantly. The LEC rat showed a 
      significantly low concentration of biliary Cu and markedly low activity of 
      ceruloplasmin (as ferroxidase). Serum Cu showed a low concentration in the 
      80-day-old LEC rats, but recovered to the control level in the 130-day-old LEC 
      rats. The abnormal accumulation of Cu may be due to the inherent reduction of 
      excretion of Cu into the bile and blood. Such deposition may be a trigger for the 
      onset of the spontaneous hepatitis occurring at 90-120 days after birth and for 
      the onset of hepatoma later.
FAU - Sugawara, N
AU  - Sugawara N
AD  - Department of Public Health, Sapporo Medical College.
FAU - Sugawara, C
AU  - Sugawara C
FAU - Sato, M
AU  - Sato M
FAU - Takahashi, H
AU  - Takahashi H
FAU - Mori, M
AU  - Mori M
LA  - eng
PT  - Journal Article
PL  - Denmark
TA  - Pharmacol Toxicol
JT  - Pharmacology & toxicology
JID - 8702180
RN  - 789U1901C5 (Copper)
RN  - 9038-94-2 (Metallothionein)
RN  - EC 1.16.3.1 (Ceruloplasmin)
SB  - IM
MH  - Animals
MH  - Ceruloplasmin/metabolism
MH  - Copper/analysis/*metabolism
MH  - Female
MH  - Hepatitis, Animal/*metabolism
MH  - Liver/*metabolism
MH  - Metallothionein/metabolism
MH  - Rats
MH  - Species Specificity
MH  - Spectrophotometry, Atomic
MH  - Tissue Distribution
EDAT- 1992/11/01 00:00
MHDA- 1992/11/01 00:01
CRDT- 1992/11/01 00:00
PHST- 1992/11/01 00:00 [pubmed]
PHST- 1992/11/01 00:01 [medline]
PHST- 1992/11/01 00:00 [entrez]
AID - 10.1111/j.1600-0773.1992.tb00554.x [doi]
PST - ppublish
SO  - Pharmacol Toxicol. 1992 Nov;71(5):321-4. doi: 10.1111/j.1600-0773.1992.tb00554.x.