PMID- 1449213
OWN - NLM
STAT- MEDLINE
DCOM- 19921228
LR  - 20190717
IS  - 0003-2700 (Print)
IS  - 0003-2700 (Linking)
VI  - 64
IP  - 19
DP  - 1992 Oct 1
TI  - Comparative study of photodissociation and surface-induced dissociation by laser 
      desorption Fourier transform mass spectrometry.
PG  - 2238-43
AB  - Photodissociation (PD) and surface-induced dissociation (SID) are compared for 
      structural analysis of several nonvolatile compounds analyzed by laser desorption 
      Fourier transform mass spectrometry (LD/FTMS). SID and PD of a porphyrin and two 
      metalloporphyrins were investigated using a variety of experimental conditions. 
      Optimum structural information is obtained from PD when parent ions are 
      irradiated for relatively long times (10-30 s) using 575-nm radiation and short 
      times (0.5-1 s) using 308- or 388-nm radiation. Shorter irradiation times in the 
      visible region resulted in less efficient production of structurally significant 
      product ions, while longer times in the ultraviolet region produced more 
      nonspecific fragment ions, apparently at the expense of more structurally 
      significant fragment ions. SID conversion efficiencies for the porphyrins are 
      estimated for collision energies from 25 to 360 eV, with maximum conversion 
      efficiency found using 62- and 115-eV collision energies for the two porphyrins 
      studied. Results from a concurrent study on the combined use of PD and SID for 
      MS/MS/MS are discussed in the context of these results. The MS3 ion spectra 
      generated by the two dissociation techniques differ more significantly than MS2 
      product ion spectra. These data suggest some general guidelines for MSn studies 
      of nonvolatile compounds analyzed by LD/FTMS, employing PD and SID for ion 
      activation.
FAU - Castoro, J A
AU  - Castoro JA
AD  - Department of Chemistry, University of California, Riverside 92521.
FAU - Nuwaysir, L M
AU  - Nuwaysir LM
FAU - Ijames, C F
AU  - Ijames CF
FAU - Wilkins, C L
AU  - Wilkins CL
LA  - eng
GR  - GM-44606/GM/NIGMS NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
SB  - IM
MH  - Fourier Analysis
MH  - Lasers
MH  - Mass Spectrometry/*instrumentation
MH  - Photochemistry
MH  - Surface Properties
EDAT- 1992/10/01 00:00
MHDA- 1992/10/01 00:01
CRDT- 1992/10/01 00:00
PHST- 1992/10/01 00:00 [pubmed]
PHST- 1992/10/01 00:01 [medline]
PHST- 1992/10/01 00:00 [entrez]
AID - 10.1021/ac00043a010 [doi]
PST - ppublish
SO  - Anal Chem. 1992 Oct 1;64(19):2238-43. doi: 10.1021/ac00043a010.