PMID- 1449245
OWN - NLM
STAT- MEDLINE
DCOM- 19921229
LR  - 20220228
IS  - 0364-5134 (Print)
IS  - 0364-5134 (Linking)
VI  - 32
IP  - 5
DP  - 1992 Nov
TI  - HTLV-I-specific cytotoxic T lymphocytes in the cerebrospinal fluid of patients 
      with HTLV-I-associated neurological disease.
PG  - 651-7
AB  - Recently, it has been shown that in patients with human T-cell lymphotropic virus 
      type I (HTLV-I)-associated neurological disease, high levels of HTLV-I-specific 
      cytotoxic T lymphocytes (CTLs) could be detected in the peripheral blood. These 
      CTLs predominantly recognized products of the pX region of HTLV-I, had a CD8+ 
      phenotype, and were human leukocyte class I restricted. Moreover, these responses 
      were not detected in asymptomatic, HTLV-I-seropositive individuals. This implied 
      a role for these CTLs in the pathogenesis of the neurological disorder associated 
      with HTLV-I. We have extended these observations by demonstrating HTLV-I-specific 
      CTLs directly from lymphocytes obtained from the cerebrospinal fluid of patients 
      with HTLV-I-associated myelopathy/tropical spastic paraparesis. Uncultured 
      cerebrospinal fluid lymphocytes were used directly as effectors on a variety of 
      targets expressing HTLV-I. These cells were lysed in a virus-specific and HLA 
      class I-restricted manner. Moreover, the cerebrospinal fluid lymphocytes were 
      sorted into purified CD8+ populations, cloned by limiting dilution, and assayed 
      for CTL activity. An exceedingly high proportion of these resultant lines were 
      shown to be cytolytic and precursor frequency analysis indicated that as many as 
      1 in 500 cells were HTLV-I-specific CTLs. The majority of these CTL lines 
      recognized HTLV-I gene products encoded within the pX region of HTLV-I. The 
      significance of these HTLV-I-specific CTLs in the central nervous system of 
      patients with HTLV-I-associated neurological disease is discussed with regard to 
      the potential role of CTLs in the pathogenesis of this disease.
FAU - Jacobson, S
AU  - Jacobson S
AD  - Neuroimmunology Branch, National Institutes of Health, Bethesda, MD 20892.
FAU - McFarlin, D E
AU  - McFarlin DE
FAU - Robinson, S
AU  - Robinson S
FAU - Voskuhl, R
AU  - Voskuhl R
FAU - Martin, R
AU  - Martin R
FAU - Brewah, A
AU  - Brewah A
FAU - Newell, A J
AU  - Newell AJ
FAU - Koenig, S
AU  - Koenig S
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Ann Neurol
JT  - Annals of neurology
JID - 7707449
RN  - 0 (CD8 Antigens)
SB  - IM
MH  - Adult
MH  - CD8 Antigens/*analysis
MH  - Clone Cells
MH  - Flow Cytometry
MH  - HTLV-I Infections/*cerebrospinal fluid/diagnosis/immunology
MH  - Humans
MH  - Male
MH  - Serologic Tests
MH  - *T-Lymphocytes, Cytotoxic
EDAT- 1992/11/01 00:00
MHDA- 1992/11/01 00:01
CRDT- 1992/11/01 00:00
PHST- 1992/11/01 00:00 [pubmed]
PHST- 1992/11/01 00:01 [medline]
PHST- 1992/11/01 00:00 [entrez]
AID - 10.1002/ana.410320508 [doi]
PST - ppublish
SO  - Ann Neurol. 1992 Nov;32(5):651-7. doi: 10.1002/ana.410320508.