PMID- 1449591
OWN - NLM
STAT- MEDLINE
DCOM- 19930107
LR  - 20191021
IS  - 0939-5075 (Print)
IS  - 0341-0382 (Linking)
VI  - 47
IP  - 9-10
DP  - 1992 Sep-Oct
TI  - A simplified combination of DNA probe preparation and fluorescence in situ 
      hybridization.
PG  - 739-47
AB  - Fluorescence in situ hybridization (FISH) has found widespread applications in 
      cytogenetics. So far the standard protocols for probe amplification (and 
      simultaneous labeling) by PCR, nick translation and in situ hybridization involve 
      different buffer systems leading to a number of time consuming washing steps even 
      before hybridization. In this manuscript we show a fast technique of a close 
      combination of DNA probe preparation and in situ hybridization (ISH). This method 
      was applied to metaphase chromosomes from human lymphocytes fixed on slides. Two 
      specific repetitive DNA probes, the pUC 1.77 DNA probe and the DYZ1 repetitive 
      DNA fraction were used, amplified and labeled in different ways. Additional 
      experiments with total genomic male human DNA as the DNA probe suggest that this 
      method may be extended to a large variety of other probes. Moreover the ISH 
      technique described does not require toxic denaturing agents, such as formamide.
FAU - Celeda, D
AU  - Celeda D
AD  - Institut fur Angewandte Physik, Universitat Heidelberg.
FAU - Bettag, U
AU  - Bettag U
FAU - Cremer, C
AU  - Cremer C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Z Naturforsch C J Biosci
JT  - Zeitschrift fur Naturforschung. C, Journal of biosciences
JID - 8912155
RN  - 0 (DNA Probes)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Chromosomes, Human/ultrastructure
MH  - DNA/*analysis/genetics
MH  - DNA Probes/*chemical synthesis
MH  - Humans
MH  - In Situ Hybridization/*methods
MH  - In Vitro Techniques
MH  - Lymphocytes/cytology
MH  - Male
MH  - Polymerase Chain Reaction/methods
MH  - Spectrometry, Fluorescence/methods
EDAT- 1992/09/01 00:00
MHDA- 1992/09/01 00:01
CRDT- 1992/09/01 00:00
PHST- 1992/09/01 00:00 [pubmed]
PHST- 1992/09/01 00:01 [medline]
PHST- 1992/09/01 00:00 [entrez]
AID - 10.1515/znc-1992-9-1017 [doi]
PST - ppublish
SO  - Z Naturforsch C J Biosci. 1992 Sep-Oct;47(9-10):739-47. doi: 
      10.1515/znc-1992-9-1017.