PMID- 14498985
OWN - NLM
STAT- MEDLINE
DCOM- 20031110
LR  - 20191108
IS  - 0047-2565 (Print)
IS  - 0047-2565 (Linking)
VI  - 32
IP  - 4-5
DP  - 2003 Aug
TI  - Expression of IFN-gamma induced CXCR3 agonist chemokines and compartmentalization 
      of CXCR3+ cells in the periphery and lymph nodes of rhesus macaques during simian 
      immunodeficiency virus infection and acquired immunodeficiency syndrome.
PG  - 247-64
AB  - Dysregulation of cytokines and chemokines during human immunodeficiency virus 1 
      (HIV-1) and simian immunodeficiency virus (SIV) infection is thought to be 
      critical in the progression of acquired immunodeficiency syndrome (AIDS). To 
      evaluate the potential role of Th1-agonist chemokines in disease progression 
      during AIDS, we assessed CXCL9/MIG and CXCL10/IP-10 expression simultaneously in 
      the periphery and lymphoid tissues of SIV-infected animals at a single-cell level 
      by flow cytometry. We optimized intracellular staining and analysis of CXCL9/MIG 
      and CXCL10/IP-10 production in human leukocyte antigen (HLA)-DR+ macaque cells by 
      flow cytometry using cross-reactive antibodies against human chemokines. We 
      observed an upregulation of CXCL9/MIG and CXCL10/IP-10 production in both the 
      periphery and lymph nodes of infected animals compared with naive controls. 
      Animals with higher viral loads had higher levels of CXCL9/MIG and CXCL10/IP-10 
      producing cells compared with animals with low viral loads. Analysis of cells 
      bearing the receptor (CXCR3) for CXCL9/MIG and CXCL10/IP-10 revealed increased 
      number of CXCR3+ cells in the lymph nodes of infected animals. Importantly, an 
      inverse correlation (P < 0.05) between CXCL9/MIG and CXCL10/IP-10 production, 
      both in the periphery and lymph nodes, and peripheral CD4+ T-cell numbers was 
      observed. These findings provide further evidence that dysregulation of Th1 
      agonist chemokines might contribute to the ultimate immunopathology during AIDS.
FAU - Sarkar, Surojit
AU  - Sarkar S
AD  - Department of Molecular Genetics and Biochemistry, University of Pittsburgh 
      School of Medicine, Pittsburg, PA 15261, USA.
FAU - Kalia, Vandana
AU  - Kalia V
FAU - Murphey-Corb, Michael
AU  - Murphey-Corb M
FAU - Montelaro, Ronald C
AU  - Montelaro RC
FAU - Reinhart, Todd A
AU  - Reinhart TA
LA  - eng
GR  - 5P01AI28243/AI/NIAID NIH HHS/United States
GR  - HL62056/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Denmark
TA  - J Med Primatol
JT  - Journal of medical primatology
JID - 0320626
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (CXCL9 protein, human)
RN  - 0 (CXCR3 protein, human)
RN  - 0 (Chemokine CXCL10)
RN  - 0 (Chemokine CXCL9)
RN  - 0 (Chemokines)
RN  - 0 (Chemokines, CXC)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Receptors, CXCR3)
RN  - 0 (Receptors, Chemokine)
RN  - 0 (Recombinant Proteins)
RN  - 6SO6U10H04 (Biotin)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Acquired Immunodeficiency Syndrome/*immunology
MH  - Animals
MH  - Antibodies, Monoclonal/immunology
MH  - Biotin
MH  - Chemokine CXCL10
MH  - Chemokine CXCL9
MH  - Chemokines/*genetics
MH  - Chemokines, CXC/genetics
MH  - Flow Cytometry
MH  - Gene Expression Regulation/*genetics
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Intercellular Signaling Peptides and Proteins/genetics
MH  - Interferon-gamma/pharmacology
MH  - Leukocytes, Mononuclear/immunology
MH  - Lymph Nodes/immunology
MH  - Macaca mulatta
MH  - Receptors, CXCR3
MH  - Receptors, Chemokine/*agonists/*immunology
MH  - Recombinant Proteins
MH  - Simian Acquired Immunodeficiency Syndrome/*immunology
MH  - Viral Load
EDAT- 2003/09/23 05:00
MHDA- 2003/11/11 05:00
CRDT- 2003/09/23 05:00
PHST- 2003/09/23 05:00 [pubmed]
PHST- 2003/11/11 05:00 [medline]
PHST- 2003/09/23 05:00 [entrez]
AID - 031 [pii]
AID - 10.1034/j.1600-0684.2003.00031.x [doi]
PST - ppublish
SO  - J Med Primatol. 2003 Aug;32(4-5):247-64. doi: 10.1034/j.1600-0684.2003.00031.x.