PMID- 14500702
OWN - NLM
STAT- MEDLINE
DCOM- 20031105
LR  - 20170214
IS  - 0022-1554 (Print)
IS  - 0022-1554 (Linking)
VI  - 51
IP  - 10
DP  - 2003 Oct
TI  - Chromosomal variations within aneuploid cancer lines.
PG  - 1343-53
AB  - Aneuploid cancers exhibit a wide spectrum of clinical aggressiveness, possibly 
      because of varying chromosome compositions. To test this, karyotypes from the 
      diploid CCD-34Lu fibroblast and the aneuploid A549 and SUIT-2 cancer lines 
      underwent fluorescence in situ hybridization (FISH) and DAPI counterstaining. The 
      number of DAPI-stained and FISH-identified chromosomes, 1-22, X,Y, as well as 
      structural abnormalities, were counted and compared using the chi(2), 
      Mann-Whitney rank sum test and the Levene's equality of variance. Virtually all 
      of the evaluable diploid CCD-34Lu karyotypes had 46 chromosomes with two 
      normal-appearing homologues. The aneuploid chromosome numbers per karyotype were 
      highly variable, averaging 62 and 72 for the A549 and SUIT-2 lines, respectively. 
      However, the A549 chromosome numbers were more narrowly distributed than the 
      SUIT-2 karyotype chromosome numbers. Furthermore, 25% of the A549 chromosomes had 
      structural abnormalities compared to only 7% of the SUIT-2 chromosomes. The 
      chromosomal compositions of the aneuploid A549 and SUIT-2 cancer lines are widely 
      divergent, suggesting that diverse genetic alterations, rather than chance, may 
      govern the chromosome makeups of aneuploid cancers.
FAU - Isaka, Takahiro
AU  - Isaka T
AD  - Department of Surgery, and the MCO Microscopy Imaging Center, Medical College of 
      Ohio, Toledo, Ohio 43614-5804, USA.
FAU - Nestor, Andrea L
AU  - Nestor AL
FAU - Takada, Tadahiro
AU  - Takada T
FAU - Allison, David C
AU  - Allison DC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Histochem Cytochem
JT  - The journal of histochemistry and cytochemistry : official journal of the 
      Histochemistry Society
JID - 9815334
RN  - 0 (Indoles)
RN  - 47165-04-8 (DAPI)
SB  - IM
MH  - Adenosarcoma/genetics
MH  - *Aneuploidy
MH  - *Chromosome Aberrations
MH  - Diploidy
MH  - Female
MH  - Fibroblasts/cytology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Indoles/chemistry
MH  - Karyotyping/methods
MH  - Lung Neoplasms/*genetics
MH  - Male
MH  - Pancreatic Neoplasms/*genetics
MH  - Staining and Labeling/methods
MH  - Tumor Cells, Cultured
EDAT- 2003/09/23 05:00
MHDA- 2003/11/06 05:00
CRDT- 2003/09/23 05:00
PHST- 2003/09/23 05:00 [pubmed]
PHST- 2003/11/06 05:00 [medline]
PHST- 2003/09/23 05:00 [entrez]
AID - 10.1177/002215540305101011 [doi]
PST - ppublish
SO  - J Histochem Cytochem. 2003 Oct;51(10):1343-53. doi: 10.1177/002215540305101011.