PMID- 14500767
OWN - NLM
STAT- MEDLINE
DCOM- 20040923
LR  - 20181113
IS  - 0022-3751 (Print)
IS  - 1469-7793 (Electronic)
IS  - 0022-3751 (Linking)
VI  - 553
IP  - Pt 2
DP  - 2003 Dec 1
TI  - Miniature synaptic transmission and BDNF modulate dendritic spine growth and form 
      in rat CA1 neurones.
PG  - 497-509
AB  - The refinement and plasticity of neuronal connections require synaptic activity 
      and neurotrophin signalling; their specific contributions and interplay are, 
      however, poorly understood. We show here that brain-derived neurotrophic factor 
      (BDNF) increased spine density in apical dendrites of CA1 pyramidal neurones in 
      organotypic slice cultures prepared from postnatal rat hippocampal slices. This 
      effect was observed also in the absence of action potentials, and even when 
      miniature synaptic transmission was inhibited with botulinum neurotoxin C 
      (BoNT/C). There were, however, marked differences in the morphology of individual 
      spines induced by BDNF across these different levels of spontaneous ongoing 
      synaptic activity. During both normal synaptic transmission, and when action 
      potentials were blocked with TTX, BDNF increased the proportion of stubby, type-I 
      spines. However, when SNARE-dependent vesicular release was inhibited with 
      BoNT/C, BDNF increased the proportion of thin, type-III spines. Our results 
      indicate that BDNF increases spine density irrespective of the levels of synaptic 
      transmission. In addition, miniature synaptic transmission provides sufficient 
      activity for the functional translation of BDNF-triggered spinogenesis into 
      clearly defined morphological spine types, favouring those spines potentially 
      responsible for coordinated Ca2+ transients thought to mediate synaptic 
      plasticity. We propose that BDNF/TrkB signalling represents a mechanism of 
      expression of both morphological and physiological homeostatic plasticity in the 
      hippocampus, leading to a more efficient synaptic information transfer across 
      widespread levels of synaptic activity.
FAU - Tyler, William J
AU  - Tyler WJ
AD  - Department of Neurobiology and Psychology, Civitan International Research Center, 
      University of Alabama at Birmingham, Birmingham, AL 35294-0021, USA.
FAU - Pozzo-Miller, Lucas
AU  - Pozzo-Miller L
LA  - eng
GR  - P30 HD038985/HD/NICHD NIH HHS/United States
GR  - R01 NS040593/NS/NINDS NIH HHS/United States
GR  - P30-HD38985/HD/NICHD NIH HHS/United States
GR  - R01-NS40593/NS/NINDS NIH HHS/United States
GR  - P01 HD038760/HD/NICHD NIH HHS/United States
GR  - P01-HD38760/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20030918
PL  - England
TA  - J Physiol
JT  - The Journal of physiology
JID - 0266262
RN  - 0 (Antigens, Surface)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (SNARE Proteins)
RN  - 0 (Syntaxin 1)
RN  - 0 (Vesicular Transport Proteins)
RN  - 4368-28-9 (Tetrodotoxin)
RN  - 77521-29-0 (alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid)
RN  - EC 3.4.24.69 (Botulinum Toxins)
RN  - FPM7829VMX (botulinum toxin type C)
SB  - IM
MH  - Action Potentials/drug effects
MH  - Animals
MH  - Antigens, Surface/metabolism
MH  - Botulinum Toxins/pharmacology
MH  - Brain-Derived Neurotrophic Factor/pharmacology/*physiology
MH  - Dendrites/classification/*metabolism
MH  - Excitatory Postsynaptic Potentials/physiology
MH  - Hippocampus/*growth & development/physiology
MH  - In Vitro Techniques
MH  - Nerve Tissue Proteins/metabolism
MH  - Neuronal Plasticity/physiology
MH  - Neurons/drug effects/*physiology
MH  - Rats
MH  - SNARE Proteins
MH  - Synaptic Transmission/*physiology
MH  - Syntaxin 1
MH  - Tetrodotoxin/pharmacology
MH  - Vesicular Transport Proteins/physiology
MH  - alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid/pharmacology
PMC - PMC2343578
EDAT- 2003/09/23 05:00
MHDA- 2004/09/24 05:00
PMCR- 2004/12/01
CRDT- 2003/09/23 05:00
PHST- 2003/09/23 05:00 [pubmed]
PHST- 2004/09/24 05:00 [medline]
PHST- 2003/09/23 05:00 [entrez]
PHST- 2004/12/01 00:00 [pmc-release]
AID - jphysiol.2003.052639 [pii]
AID - 10.1113/jphysiol.2003.052639 [doi]
PST - ppublish
SO  - J Physiol. 2003 Dec 1;553(Pt 2):497-509. doi: 10.1113/jphysiol.2003.052639. Epub 
      2003 Sep 18.