PMID- 14501050
OWN - NLM
STAT- MEDLINE
DCOM- 20040220
LR  - 20190901
IS  - 1064-3745 (Print)
IS  - 1064-3745 (Linking)
VI  - 237
DP  - 2004
TI  - In vivo adenoviral-mediated gene transfer of the beta ARKct to study the role of 
      G beta gamma in arterial restenosis.
PG  - 181-92
AB  - A large body of in vitro studies has helped to elucidate intracellular pathways 
      that lead to mitogenic signaling in vascular smooth muscle (VSM) cells. However, 
      a limitation of these studies is that they fail to test the in vivo physiological 
      significance especially because VSM proliferation, in the forms of intimal 
      hyperplasia and restenosis, is an important clinical problem. The recent advent 
      of adenoviral gene transfer technology has made possible to test the in vivo 
      effects of specific molecular modulations of intracellular signal transduction 
      pathways on physiological responses. For example, in VSM, adenoviruses can be 
      delivered to the vessel wall to determine a gene/protein's role in proliferative 
      responses to vascular injury. This technology, once standardized and rendered 
      safe for human applications, will be the basis of gene therapy and molecular 
      medicine. Several exemplary applications have now been generated in the vascular 
      system, including the use of an adenovirus containing the carboxyl-terminus of 
      the beta-adrenergic receptor kinase (beta ARKct), which binds to the beta 
      gamma-subunits of activated heterotrimeric G proteins (G beta gamma), to study 
      the in vivo role of G beta gamma in VSM intimal hyperplasia after vascular injury 
      that leads to restenosis.
FAU - Iaccarino, Guido
AU  - Iaccarino G
AD  - Dipartmento di Medicina Clinica, Scienze Cardiovasculari ed Immunologiche, 
      Federico II University, Naples, Italy.
FAU - Koch, Walter J
AU  - Koch WJ
LA  - eng
PT  - Journal Article
PT  - Review
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Carrier Proteins)
RN  - 0 (GTP-Binding Protein beta Subunits)
RN  - 0 (GTP-Binding Protein gamma Subunits)
RN  - 0 (Peptides)
RN  - 0 (Recombinant Proteins)
RN  - 0 (beta-adrenergic receptor kinase inhibitory peptide)
SB  - IM
MH  - Adenoviridae/*genetics
MH  - Animals
MH  - Carotid Artery Injuries/metabolism
MH  - Carrier Proteins/*genetics
MH  - Cell Line
MH  - Coronary Restenosis/*metabolism
MH  - GTP-Binding Protein beta Subunits/*metabolism
MH  - GTP-Binding Protein gamma Subunits/*metabolism
MH  - *Gene Transfer Techniques
MH  - Humans
MH  - Kidney/cytology
MH  - *Peptides
MH  - Rats
MH  - *Recombinant Proteins
MH  - Signal Transduction
RF  - 18
EDAT- 2003/09/23 05:00
MHDA- 2004/02/21 05:00
CRDT- 2003/09/23 05:00
PHST- 2003/09/23 05:00 [pubmed]
PHST- 2004/02/21 05:00 [medline]
PHST- 2003/09/23 05:00 [entrez]
AID - 1-59259-430-1-181 [pii]
AID - 10.1385/1-59259-430-1:181 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2004;237:181-92. doi: 10.1385/1-59259-430-1:181.