PMID- 14504748
OWN - NLM
STAT- MEDLINE
DCOM- 20040305
LR  - 20221207
IS  - 0040-5752 (Print)
IS  - 0040-5752 (Linking)
VI  - 108
IP  - 2
DP  - 2004 Jan
TI  - Localization of jointless-2 gene in the centromeric region of tomato chromosome 
      12 based on high resolution genetic and physical mapping.
PG  - 190-6
AB  - Abscission is a universal process whereby plants shed their organs, such as 
      flowers, fruit and leaves. In tomato, the non-allelic mutations jointless and 
      jointless-2 have been discovered as recessive mutations that completely suppress 
      the formation of pedicel abscission zones. A high resolution genetic map of 
      jointless-2 was constructed using 1,122 jointless F2 plants. Restriction fragment 
      length polymorphism (RFLP) marker RPD140 completely co-segregated with the 
      jointless-2 locus and mapped in a 2.4 cM interval between RFLP markers CD22 and 
      TG618. To chromosome walk to jointless-2, all three markers were used to screen a 
      bacterial artificial chromosome (BAC) library and contigs were developed. 
      Intensive efforts to expand and merge the BAC contigs were unsuccessful because 
      of the highly repetitive sequence content on the distal ends of each contig. To 
      determine the physical distance between and the orientation of the three contigs, 
      we used high resolution pachytene fluorescence in situ hybridization (FISH) 
      mapping. The RPD140 contig was positioned in the centromeric region of chromosome 
      12 between two large pericentric heterochromatin blocks, about 50 Mb from the 
      TG618 contig on the short arm and 10 Mb from the CD22 contig on the long arm, 
      respectively. Based on high resolution genetic and physical mapping, we conclude 
      that the jointless-2 gene is located within or near the chromosome 12 centromere 
      where 1 cM is approximately 25 Mb in length.
FAU - Budiman, M A
AU  - Budiman MA
AD  - Genomics Institute, Clemson University, Clemson, SC 29634, USA.
FAU - Chang, S-B
AU  - Chang SB
FAU - Lee, S
AU  - Lee S
FAU - Yang, T J
AU  - Yang TJ
FAU - Zhang, H-B
AU  - Zhang HB
FAU - de Jong, H
AU  - de Jong H
FAU - Wing, R A
AU  - Wing RA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20030920
PL  - Germany
TA  - Theor Appl Genet
JT  - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
JID - 0145600
RN  - 0 (DNA, Plant)
RN  - 0 (Genetic Markers)
RN  - 0 (J protein, Lycopersicon esculentum)
RN  - 0 (MADS Domain Proteins)
RN  - 0 (Plant Proteins)
SB  - IM
MH  - Centromere/*genetics
MH  - Chromosome Walking
MH  - Chromosomes, Artificial, Bacterial
MH  - Chromosomes, Plant/*genetics
MH  - DNA, Plant/genetics
MH  - Genetic Markers
MH  - Hybridization, Genetic
MH  - In Situ Hybridization, Fluorescence
MH  - Solanum lycopersicum/*genetics
MH  - MADS Domain Proteins/*genetics
MH  - *Physical Chromosome Mapping
MH  - Plant Proteins/*genetics
MH  - Polymorphism, Restriction Fragment Length
EDAT- 2003/09/25 05:00
MHDA- 2004/03/06 05:00
CRDT- 2003/09/25 05:00
PHST- 2003/04/09 00:00 [received]
PHST- 2003/08/12 00:00 [accepted]
PHST- 2003/09/25 05:00 [pubmed]
PHST- 2004/03/06 05:00 [medline]
PHST- 2003/09/25 05:00 [entrez]
AID - 10.1007/s00122-003-1429-3 [doi]
PST - ppublish
SO  - Theor Appl Genet. 2004 Jan;108(2):190-6. doi: 10.1007/s00122-003-1429-3. Epub 
      2003 Sep 20.