PMID- 14506697
OWN - NLM
STAT- MEDLINE
DCOM- 20031204
LR  - 20041117
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 38
IP  - 3
DP  - 2003 Nov
TI  - Further cytogenetic characterization of multiple myeloma confirms that 14q32 
      translocations are a very rare event in hyperdiploid cases.
PG  - 234-9
AB  - Translocations involving the immunoglobulin heavy-chain genes are frequent in 
      multiple myeloma (MM), which can be separated into two groups according to the 
      chromosome number pattern. 14q32 translocations 14q32t are more frequent in 
      hypodiploid than in hyperdiploid karyotypes. However, conventional cytogenetics 
      (CC) misses cryptic translocations, especially t(4;14)(p16;q32). Furthermore, 
      recent interphase fluorescence in situ hybridization (FISH) studies found 14q32t 
      in as many as 75% of MM cases. To identify in which CC group we failed to detect 
      translocations, we designed a study by use of FISH with a dual-color IGH probe on 
      previously R-banded metaphase cells, allowing the detection of both 14q32t and 
      overall chromosomal abnormalities, in a new series of 55 MM with abnormal 
      karyotypes: 4/29 hyperdiploid (14%) and 19/26 hypodiploid (73%) cases had a 
      14q32t. The t(4;14) was found in 2 hyperdiploid (7%) and 10 hypodiploid (39%) 
      cases. We therefore confirm that 14q32t are much more frequent in hypodiploid 
      than in hyperdiploid MM (P<0.0001) and that cryptic t(4;14)(p16;q32) is strongly 
      associated with hypodiploid karyotypes (P<0.01). Through the use of this reliable 
      assay, only 42% of MM had 14q32t.
CI  - Copyright 2003 Wiley-Liss, Inc.
FAU - Smadja, Nicole Veronique
AU  - Smadja NV
AD  - Cytogenetic Research Laboratory, Hopital Saint Antoine, AP-HP, Paris, France. 
      veronique.smadja@sat.ap-hop-paris.fr
FAU - Leroux, Dominique
AU  - Leroux D
FAU - Soulier, Jean
AU  - Soulier J
FAU - Dumont, Sylvie
AU  - Dumont S
FAU - Arnould, Catherine
AU  - Arnould C
FAU - Taviaux, Sylvie
AU  - Taviaux S
FAU - Taillemite, Jean Louis
AU  - Taillemite JL
FAU - Bastard, Christian
AU  - Bastard C
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
SB  - IM
MH  - Chromosome Aberrations/*classification
MH  - Chromosomes, Human, Pair 14/*genetics
MH  - Cytogenetic Analysis/*methods
MH  - *Diploidy
MH  - Female
MH  - Humans
MH  - Karyotyping
MH  - Male
MH  - Middle Aged
MH  - Multiple Myeloma/*genetics
MH  - Translocation, Genetic/*genetics
MH  - Trisomy/genetics
EDAT- 2003/09/25 05:00
MHDA- 2003/12/05 05:00
CRDT- 2003/09/25 05:00
PHST- 2003/09/25 05:00 [pubmed]
PHST- 2003/12/05 05:00 [medline]
PHST- 2003/09/25 05:00 [entrez]
AID - 10.1002/gcc.10275 [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 2003 Nov;38(3):234-9. doi: 10.1002/gcc.10275.