PMID- 14506699
OWN - NLM
STAT- MEDLINE
DCOM- 20031204
LR  - 20071114
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 38
IP  - 3
DP  - 2003 Nov
TI  - Molecular cytogenetic characterization of the KG-1 and KG-1a acute myeloid 
      leukemia cell lines by use of spectral karyotyping and fluorescence in situ 
      hybridization.
PG  - 249-52
AB  - The KG-1 cell line, established from bone marrow cells of a patient with 
      erythroleukemia evolving to acute myelogenous leukemia, and its less 
      differentiated variant, KG-1a, are widely used in research worldwide. However, to 
      our knowledge, neither cell line was studied by use of molecular-cytogenetic 
      techniques such as spectral karyotyping (SKY) and fluorescence in situ 
      hybridization (FISH). Our G-banding, SKY, and FISH analyses revealed a complex 
      karyotype with a pseudodiploid modal chromosome number in both the KG-1 and KG-1a 
      cell lines. The lines shared several identical structural aberrations, including 
      der(4)t(4;8), del(7q), der(8)t(8;12), idic(8)(p11), der(17)t(5;17), and 
      der(20)t(12;20), but also differed with regard to other chromosome 
      rearrangements. In contrast to KG-1, the KG-1a line lost one of the two copies of 
      idic(8)(p11) present in KG-1 cells and gained a der(8;22)(q24;q13), an 
      i(11)(q10), and a der(19)t(14;19)(q13;q13.4). Notably, we have shown that the 
      KG-1 cells harbor a partial hexasomy of the long arm of chromosome 8, which may 
      explain in part the previously reported significantly higher rate of formation of 
      the AML1-ETO fusion gene in KG-1 cells subjected to high-dose gamma irradiation 
      compared with the rates of formation of the BCR-ABL or the DEK-CAN fusion gene. 
      Our detailed description of chromosome rearrangements in KG-1 and KG-1a will be 
      useful for the cytogenetic authentication of the lines, and provide clues as to 
      the regions of the genome that could be studied further to explain the 
      differences in phenotypic properties between KG-1 and KG-1a cells.
CI  - Copyright 2003 Wiley-Liss, Inc.
FAU - Mrozek, Krzysztof
AU  - Mrozek K
AD  - Division of Hematology and Oncology, and the Comprehensive Cancer Center, The 
      Ohio State University, Columbus, Ohio, USA. Mrozek-1@medctr.osu.edu
FAU - Tanner, Stephan M
AU  - Tanner SM
FAU - Heinonen, Kristiina
AU  - Heinonen K
FAU - Bloomfield, Clara D
AU  - Bloomfield CD
LA  - eng
GR  - 5P30 CA-16058/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
SB  - IM
MH  - Acute Disease
MH  - Cell Line, Tumor
MH  - Chromosome Aberrations
MH  - Chromosome Banding/methods
MH  - Chromosome Painting/methods
MH  - Cytogenetic Analysis/*methods
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Karyotyping
MH  - Leukemia, Myeloid/*genetics
MH  - Male
EDAT- 2003/09/25 05:00
MHDA- 2003/12/05 05:00
CRDT- 2003/09/25 05:00
PHST- 2003/09/25 05:00 [pubmed]
PHST- 2003/12/05 05:00 [medline]
PHST- 2003/09/25 05:00 [entrez]
AID - 10.1002/gcc.10274 [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 2003 Nov;38(3):249-52. doi: 10.1002/gcc.10274.