PMID- 14507902
OWN - NLM
STAT- MEDLINE
DCOM- 20031016
LR  - 20190607
IS  - 0146-0404 (Print)
IS  - 0146-0404 (Linking)
VI  - 44
IP  - 10
DP  - 2003 Oct
TI  - In vivo expression of neurotrophins and neurotrophin receptors is conserved in 
      adult porcine retina in vitro.
PG  - 4532-41
AB  - PURPOSE: To characterize and compare the expression of neurotrophins (NTs) and 
      their receptors within adult porcine retinal ganglion cells (RGCs) in vivo and in 
      vitro. METHODS: The distribution of nerve growth factor (NGF), brain-derived 
      neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and -4 (NT-4), and their 
      high-affinity receptors TrkA, TrkB, TrkC and low-affinity receptor p75, was 
      analyzed in adult porcine retinal sections by immunohistochemistry. In addition, 
      adult porcine retinas were dissociated and cultured in four different conditions: 
      control, semipure RGCs, supplemented with BDNF, or seeded on Muller glia feeder 
      layers. Double immunostaining was performed with antibodies to NTs or their 
      receptors combined with neurofilament antibody to identify RGCs in culture. 
      RESULTS: In vivo, immunolabeling of NGF was very faint, BDNF was especially 
      prominent in RGCs and inner nuclear layer cells, NT-3 stained widespread nuclei, 
      and NT-4 was undetectable. TrkA immunoreactivity was visible in the nerve fiber 
      layer, TrkB staining was within RGC bodies, TrkC was undetectable, and p75 was 
      widely expressed across the retina, within the Muller glia. Expression of 
      neurotrophins and their receptors was maintained in all four models of adult RGCs 
      in vitro, showing that expression was not influenced by substrate or culture 
      conditions. We observed prominent staining of TrkA within growth cones, and a 
      clear expression of p75 within a subpopulation of RGCs in vitro. CONCLUSIONS: 
      These findings demonstrate that the expression of NTs and their receptors within 
      adult porcine RGCs is maintained in vitro, under conditions of limited 
      interaction with neighboring neurons and deprived of afferent inputs and target 
      tissue. TrkA may be involved in regeneration of nerve terminals.
FAU - Garcia, Monica
AU  - Garcia M
AD  - University of Pais Vasco, Faculty of Medicine, Department of Cellular Biology, 
      Vizcaya, Spain.
FAU - Forster, Valerie
AU  - Forster V
FAU - Hicks, David
AU  - Hicks D
FAU - Vecino, Elena
AU  - Vecino E
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Receptors, Nerve Growth Factor)
SB  - IM
MH  - Animals
MH  - Cell Culture Techniques
MH  - Fluorescent Antibody Technique, Indirect
MH  - Nerve Growth Factors/*metabolism
MH  - Receptors, Nerve Growth Factor/*metabolism
MH  - Retina/*metabolism
MH  - Retinal Ganglion Cells/metabolism
MH  - Swine
EDAT- 2003/09/26 05:00
MHDA- 2003/10/17 05:00
CRDT- 2003/09/26 05:00
PHST- 2003/09/26 05:00 [pubmed]
PHST- 2003/10/17 05:00 [medline]
PHST- 2003/09/26 05:00 [entrez]
AID - 10.1167/iovs.03-0419 [doi]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 2003 Oct;44(10):4532-41. doi: 10.1167/iovs.03-0419.