PMID- 14507981
OWN - NLM
STAT- MEDLINE
DCOM- 20031014
LR  - 20200225
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Print)
IS  - 0270-6474 (Linking)
VI  - 23
IP  - 25
DP  - 2003 Sep 24
TI  - Vaccination with dendritic cells pulsed with peptides of myelin basic protein 
      promotes functional recovery from spinal cord injury.
PG  - 8808-19
AB  - Injury-induced self-destructive processes cause significant functional loss after 
      incomplete spinal cord injury (SCI). Cellular elements of both the innate 
      (macrophage) and the adaptive (T-cell) immune response can, if properly activated 
      and controlled, promote post-traumatic regrowth and protection after SCI. 
      Dendritic cells (DCs) trigger activation of effector and regulatory T-cells, 
      providing a link between the functions of the innate and the adaptive immune 
      systems. They also initiate and control the body's response to pathogenic agents 
      and regulate immune responses to both foreign and self-antigens. Here we show 
      that post-injury injection of bone marrow-derived DCs pulsed with 
      encephalitogenic or nonencephalitogenic peptides derived from myelin basic 
      protein, when administered (either systemically or locally by injection into the 
      lesion site) up to 12 d after the injury, led to significant and pronounced 
      recovery from severe incomplete SCI. No significant protection was seen in DC 
      recipients deprived of mature T-cells. Flow cytometry, RT-PCR, and proliferation 
      assays indicated that the DCs prepared and used here were mature and immunogenic. 
      Taken together, the results suggest that the DC-mediated neuroprotection was 
      achieved via the induction of a systemic T-cell-dependent immune response. Better 
      preservation of neural tissue and diminished formation of cysts and scar tissue 
      accompanied the improved functional recovery in DC-treated rats. The use of 
      antigen-specific DCs may represent an effective way to obtain, via transient 
      induction of an autoimmune response, the maximal benefit of immune-mediated 
      repair and maintenance as well as protection against self-destructive compounds.
FAU - Hauben, Ehud
AU  - Hauben E
AD  - Department of Neurobiology, The Weizmann Institute of Science, 76100 Rehovot, 
      Israel.
FAU - Gothilf, Amalia
AU  - Gothilf A
FAU - Cohen, Avi
AU  - Cohen A
FAU - Butovsky, Oleg
AU  - Butovsky O
FAU - Nevo, Uri
AU  - Nevo U
FAU - Smirnov, Igor
AU  - Smirnov I
FAU - Yoles, Eti
AU  - Yoles E
FAU - Akselrod, Solange
AU  - Akselrod S
FAU - Schwartz, Michal
AU  - Schwartz M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Myelin Basic Protein)
RN  - 0 (Peptide Fragments)
SB  - IM
MH  - Animals
MH  - Autoimmunity/immunology
MH  - Cells, Cultured
MH  - Dendritic Cells/cytology/drug effects/physiology/*transplantation
MH  - Disease Models, Animal
MH  - Encephalomyelitis, Autoimmune, Experimental/immunology/prevention & control
MH  - Magnetic Resonance Imaging
MH  - Motor Activity/physiology
MH  - Myelin Basic Protein/chemistry/*immunology
MH  - Nerve Regeneration/immunology
MH  - Peptide Fragments/immunology/*pharmacology
MH  - Rats
MH  - Rats, Inbred Lew
MH  - Rats, Sprague-Dawley
MH  - Recovery of Function/drug effects
MH  - Spinal Cord/immunology/pathology
MH  - Spinal Cord Injuries/diagnosis/immunology/*therapy
MH  - Treatment Outcome
MH  - Vaccination/*methods
PMC - PMC6740409
EDAT- 2003/09/26 05:00
MHDA- 2003/10/15 05:00
PMCR- 2004/03/24
CRDT- 2003/09/26 05:00
PHST- 2003/09/26 05:00 [pubmed]
PHST- 2003/10/15 05:00 [medline]
PHST- 2003/09/26 05:00 [entrez]
PHST- 2004/03/24 00:00 [pmc-release]
AID - 23/25/8808 [pii]
AID - 10.1523/JNEUROSCI.23-25-08808.2003 [doi]
PST - ppublish
SO  - J Neurosci. 2003 Sep 24;23(25):8808-19. doi: 10.1523/JNEUROSCI.23-25-08808.2003.