PMID- 14509710
OWN - NLM
STAT- MEDLINE
DCOM- 20031028
LR  - 20061115
IS  - 0043-1354 (Print)
IS  - 0043-1354 (Linking)
VI  - 37
IP  - 13
DP  - 2003 Jul
TI  - Molecular characterization of the microbial community structure in two activated 
      sludge systems for the advanced treatment of domestic effluents.
PG  - 3224-32
AB  - Although activated sludge systems with enhanced biological phosphorus removal 
      (EBPR) represent state-of-the-art technology for phosphate removal from 
      wastewater it is still unknown which species of bacteria are responsible for the 
      EBPR process. The aim of this study was to compare the bacterial composition of 
      activated sludge from two laboratory plants with different modes of operation, 
      anoxic/oxic- (EBPR, no nitrification) and Phoredox-system (EBPR, nitrification 
      and denitrification) with particular emphasis on microorganisms responsible for 
      EBPR process. In addition to fluorescence in situ hybridization (FISH), we 
      applied further rRNA-based molecular techniques like terminal 
      restriction-fragment length polymorphism analysis and comparative 16S rDNA 
      analysis to yield additional information and to verify the results from FISH 
      analysis, like e.g. for the identification of polyphosphate accumulating 
      organisms (PAO). Despite the different modes of operation only minor differences 
      in the bacterial composition were detected by FISH analysis based on the probes 
      used in this study. In contrast T-RFLP analysis yielded characteristic community 
      fingerprints for each of the investigated plants and comparative 16S rDNA 
      analysis indicated highly diverse microbial communities in both plants suggesting 
      substantial differences in the microbial structure. The results obtained by FISH 
      analysis with specific probes for PAOs support the presumption that not only one 
      specific organism is responsible for the EBPR. In our case Tetrasphaera spp. 
      dominated the PAO community, but other possible PAOs, like Microlunatus spp. and 
      members of the Rhodocyclus group, were also detected.
FAU - Eschenhagen, Martin
AU  - Eschenhagen M
AD  - Institute of Microbiology, Dresden University of Technology, Mommsenstrasse 13, 
      Dresden D-01169, Germany. eschen@rcs.urz.tu-dresden.de
FAU - Schuppler, Markus
AU  - Schuppler M
FAU - Roske, Isolde
AU  - Roske I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Water Res
JT  - Water research
JID - 0105072
RN  - 0 (DNA, Bacterial)
RN  - 0 (Phosphates)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Sewage)
SB  - IM
MH  - *Bacteria
MH  - Bioreactors
MH  - DNA, Bacterial/*analysis
MH  - In Situ Hybridization, Fluorescence
MH  - Phosphates/*metabolism
MH  - Polymorphism, Restriction Fragment Length
MH  - Population Dynamics
MH  - RNA, Ribosomal, 16S/*analysis
MH  - Sewage/microbiology
MH  - *Waste Disposal, Fluid
EDAT- 2003/09/27 05:00
MHDA- 2003/10/29 05:00
CRDT- 2003/09/27 05:00
PHST- 2003/09/27 05:00 [pubmed]
PHST- 2003/10/29 05:00 [medline]
PHST- 2003/09/27 05:00 [entrez]
AID - S0043-1354(03)00136-2 [pii]
AID - 10.1016/S0043-1354(03)00136-2 [doi]
PST - ppublish
SO  - Water Res. 2003 Jul;37(13):3224-32. doi: 10.1016/S0043-1354(03)00136-2.