PMID- 14510696
OWN - NLM
STAT- MEDLINE
DCOM- 20031212
LR  - 20230124
IS  - 1600-6135 (Print)
IS  - 1600-6135 (Linking)
VI  - 3
IP  - 10
DP  - 2003 Oct
TI  - Adenovirus transduction induces expression of multiple chemokines and chemokine 
      receptors in murine beta cells and pancreatic islets.
PG  - 1230-41
AB  - Adenoviral vectors are highly efficient for transferring genes to islets. 
      However, the inflammatory and immune responses stimulated by adenovirus may be 
      detrimental to islet survival. Given the role of chemokines and their receptors 
      in inflammation, we analyzed their expression in isolated murine islets, in a 
      murine beta cell line and in syngeneic islet grafts after adenovirus transduction 
      (AdRSVLacZ). AdRSVLacZ transduction enhanced and induced the expression of a 
      variety of chemokines. Transduced syngeneic transplanted islets showed 
      significantly enhanced expression of multiple chemokines and receptors, including 
      monocyte chemoattractant protein-1 (MCP-1), CC chemokine receptor 2 (CCR2) and 
      regulated upon activation, normal T cell expressed and secreted (RANTES), 
      compared with untransduced islet grafts. AdRSVLacZ-transduced islet grafts had 
      significant mononuclear infiltrates, and in situ hybridization demonstrated 
      intragraft expression of MCP-1, CCR2 and RANTES. Although adenovirus transduction 
      did not impair in vitro insulin secretion, diabetes was reversed in only one of 
      six recipients of a marginal mass of AdRSVLacZ-transduced islets, compared with 
      six of six control recipients. In conclusion, multiple chemokines and chemokine 
      receptors are expressed by murine islets constitutively and in response to 
      adenovirus transduction. Adenovirus transduction impairs engraftment of marginal 
      mass of transplanted islets. This is not because of direct vector toxicity of 
      islet secretory capacity, but may be related to host innate immunity in response 
      to adenovirus vector.
FAU - Zhang, Nan
AU  - Zhang N
AD  - Recanati-Miller Transplantation Institute, Carl C Icahn Center for Gene Therapy 
      and Molecular Medicine, Mount Sinai School of Medicine, New York, NY, USA.
FAU - Schroppel, Bernd
AU  - Schroppel B
FAU - Chen, Dongmei
AU  - Chen D
FAU - Fu, Shuang
AU  - Fu S
FAU - Hudkins, Kelly L
AU  - Hudkins KL
FAU - Zhang, Haojiang
AU  - Zhang H
FAU - Murphy, Barbara M
AU  - Murphy BM
FAU - Sung, Randall S
AU  - Sung RS
FAU - Bromberg, Jonathan S
AU  - Bromberg JS
LA  - eng
GR  - AI42840/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Transplant
JT  - American journal of transplantation : official journal of the American Society of 
      Transplantation and the American Society of Transplant Surgeons
JID - 100968638
RN  - 0 (Ccr2 protein, mouse)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL5)
RN  - 0 (Chemokines)
RN  - 0 (DNA, Complementary)
RN  - 0 (Insulin)
RN  - 0 (Receptors, CCR2)
RN  - 0 (Receptors, Chemokine)
RN  - 63231-63-0 (RNA)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Adenoviridae/*genetics
MH  - Animals
MH  - Chemokine CCL2/biosynthesis
MH  - Chemokine CCL5/biosynthesis
MH  - Chemokines/*biosynthesis/genetics/metabolism
MH  - DNA, Complementary/metabolism
MH  - Gene Transfer Techniques
MH  - Genetic Vectors
MH  - Glucose/metabolism
MH  - In Situ Hybridization
MH  - Insulin/metabolism
MH  - Insulin Secretion
MH  - Islets of Langerhans/*metabolism
MH  - Islets of Langerhans Transplantation/methods
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Oligonucleotide Array Sequence Analysis
MH  - RNA/metabolism
MH  - Receptors, CCR2
MH  - Receptors, Chemokine/*biosynthesis/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Time Factors
EDAT- 2003/09/27 05:00
MHDA- 2003/12/13 05:00
CRDT- 2003/09/27 05:00
PHST- 2003/09/27 05:00 [pubmed]
PHST- 2003/12/13 05:00 [medline]
PHST- 2003/09/27 05:00 [entrez]
AID - S1600-6135(22)07635-3 [pii]
AID - 10.1046/j.1600-6143.2003.00215.x [doi]
PST - ppublish
SO  - Am J Transplant. 2003 Oct;3(10):1230-41. doi: 10.1046/j.1600-6143.2003.00215.x.