PMID- 14510846
OWN - NLM
STAT- MEDLINE
DCOM- 20040316
LR  - 20191108
IS  - 1462-2912 (Print)
IS  - 1462-2912 (Linking)
VI  - 5
IP  - 10
DP  - 2003 Oct
TI  - Marine prosthecate bacteria involved in the ennoblement of stainless steel.
PG  - 925-32
AB  - Ennoblement, a phenomenon in which open-circuit potential is elevated to a noble 
      value, triggers metal corrosion in the environment and is considered to be 
      biologically catalysed. This study investigated the involvement of marine 
      microorganisms in the ennoblement of stainless steel coupons in sea water pumped 
      from Kamaishi Bay. Scanning electron microscopy (SEM) showed significant 
      attachment of prosthecate bacteria on the surfaces of stainless steel coupons in 
      the course of ennoblement. In denaturing gradient gel electrophoresis (DGGE) 
      analyses of polymerase chain reaction-amplified bacterial 16S rDNA fragments, 
      several major bands were detected from the surface of the ennobled coupons, 
      including those affiliated with the alpha and gamma subclasses of the 
      Proteobacteria. After these observations, bacterial strains were isolated from 
      the surface of the ennobled coupon. The 16S rDNA analysis revealed that a 
      bacterial isolate (designated PWB3) corresponded to a major DGGE band 
      representing an alpha-Proteobacterial population; a database analysis showed that 
      its closest relative was Rhodobium spp., albeit with low homology ( approximately 
      89%). SEM indicated that this bacterium was a prosthecate bacterium that was 
      morphologically similar to those observed on the ennobled coupons. In pure 
      culture of strain PWB3, stainless steel coupons were ennobled when the culture 
      was supplemented with MnCl2. Manganese was recovered from the surface of the 
      ennobled coupons after treatment with a reducing agent. These results suggest 
      that the attachment of manganese-oxidizing prosthecate bacteria triggered the 
      ennoblement of stainless steel in Kamaishi Bay sea water.
FAU - Baker, Paul W
AU  - Baker PW
AD  - Laboratory of Applied Microbiology, Marine Biotechnology Institute, 3-75-1 Heita, 
      Kamaishi, Iwate 026-0001, Japan.
FAU - Ito, Kimio
AU  - Ito K
FAU - Watanabe, Kazuya
AU  - Watanabe K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Environ Microbiol
JT  - Environmental microbiology
JID - 100883692
RN  - 0 (DNA, Ribosomal)
RN  - 12597-68-1 (Stainless Steel)
SB  - IM
MH  - Bacteria/classification/genetics/isolation & purification/*metabolism
MH  - Corrosion
MH  - DNA, Ribosomal/analysis
MH  - Humans
MH  - Microscopy, Electron, Scanning
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Seawater/chemistry/*microbiology
MH  - Stainless Steel/*chemistry
EDAT- 2003/09/27 05:00
MHDA- 2004/03/18 05:00
CRDT- 2003/09/27 05:00
PHST- 2003/09/27 05:00 [pubmed]
PHST- 2004/03/18 05:00 [medline]
PHST- 2003/09/27 05:00 [entrez]
AID - 489 [pii]
AID - 10.1046/j.1462-2920.2003.00489.x [doi]
PST - ppublish
SO  - Environ Microbiol. 2003 Oct;5(10):925-32. doi: 10.1046/j.1462-2920.2003.00489.x.