PMID- 14511108
OWN - NLM
STAT- MEDLINE
DCOM- 20031106
LR  - 20190630
IS  - 0022-3042 (Print)
IS  - 0022-3042 (Linking)
VI  - 87
IP  - 2
DP  - 2003 Oct
TI  - Pharmacokinetics of systemically administered tyrosine: a comparison of serum, 
      brain tissue and in vivo microdialysate levels in the rat.
PG  - 310-7
AB  - Tyrosine uptake has been reported to differ across brain regions. However, such 
      studies have typically been conducted over brief intervals and in anesthetized 
      rats; anesthesia itself affects amino acid transport across the blood-brain 
      barrier. To address these concerns, serum, brain tissue and in vivo 
      microdialysate tyrosine levels were compared for 0-3 h after administration of 
      tyrosine [0.138-1.10 mmol/kg intraperitoneally (i.p.)] to groups of awake rats. 
      Serum and brain tissue tyrosine levels increased linearly with respect to dose. 
      Basal tissue tyrosine levels varied significantly across brain regions [medial 
      prefrontal cortex (MPFC), striatum, hypothalamus, and cerebellum], but the rate 
      of tyrosine uptake was similar for hypothalamus, striatum and MPFC. For brain 
      regions in which tyrosine levels in both microdialysate and tissue were assayed, 
      namely MPFC and striatum, there was a high degree of correlation between tyrosine 
      levels in tissue and in microdialysate. Increasing brain tyrosine levels had no 
      effect on DA levels in MPFC microdialysate. We conclude that (i) regional 
      differences in the response of dopamine neurons to systemic tyrosine 
      administration cannot be attributed to pharmacokinetic factors; (ii) in vivo 
      microdialysate provides an excellent index over time and across a wide range of 
      tyrosine doses, of brain tissue tyrosine levels; and (iii) increases in brain 
      tyrosine levels do not affect basal DA release in the MPFC.
FAU - Bongiovanni, Rodolfo
AU  - Bongiovanni R
AD  - Mental Health Care Line, Louis Stokes Veterans Affairs Medical Center, Cleveland, 
      Ohio 44141, USA. rbongi4333@aol.com
FAU - Yamamoto, Bryan K
AU  - Yamamoto BK
FAU - Simpson, Catherine
AU  - Simpson C
FAU - Jaskiw, George E
AU  - Jaskiw GE
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - England
TA  - J Neurochem
JT  - Journal of neurochemistry
JID - 2985190R
RN  - 42HK56048U (Tyrosine)
SB  - IM
MH  - Animals
MH  - Brain/*metabolism
MH  - Brain Chemistry
MH  - Cerebellum/metabolism
MH  - Corpus Striatum/metabolism
MH  - Dose-Response Relationship, Drug
MH  - Extracellular Space/chemistry/metabolism
MH  - Hypothalamus/metabolism
MH  - Injections, Intraperitoneal
MH  - Kinetics
MH  - Male
MH  - Microdialysis
MH  - Prefrontal Cortex/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Tyrosine/administration & dosage/blood/*pharmacokinetics
EDAT- 2003/09/27 05:00
MHDA- 2003/11/07 05:00
CRDT- 2003/09/27 05:00
PHST- 2003/09/27 05:00 [pubmed]
PHST- 2003/11/07 05:00 [medline]
PHST- 2003/09/27 05:00 [entrez]
AID - 2007 [pii]
AID - 10.1046/j.1471-4159.2003.02007.x [doi]
PST - ppublish
SO  - J Neurochem. 2003 Oct;87(2):310-7. doi: 10.1046/j.1471-4159.2003.02007.x.