PMID- 14511315
OWN - NLM
STAT- MEDLINE
DCOM- 20031205
LR  - 20190815
IS  - 0953-816X (Print)
IS  - 0953-816X (Linking)
VI  - 18
IP  - 6
DP  - 2003 Sep
TI  - Bicuculline induces synapse formation on primary cultured accessory olfactory 
      bulb neurons.
PG  - 1343-52
AB  - To investigate the roles of the GABAergic inhibitory system of accessory 
      olfactory bulb (AOB) in pheromonal memory formation, we have developed a primary 
      culture system of AOB neurons, which had numerous excitatory and inhibitory 
      synapses. Using this culture system of AOB neurons, we examined the correlation 
      in rats between neuronal excitation and synaptic morphology by 
      bicuculline-induced disinhibition of cultured AOB neurons. The exposure to 
      bicuculline induced long-lasting oscillatory changes in the intracellular calcium 
      level ([Ca2+]in) of cultured non-GABAergic multipolar neurons, which were 
      identified as mitral/tufted cells (MT cells). These MT cells exhibited the 
      appearance of dendritic filopodia structures after a 10-min treatment with 
      bicuculline. By labelling presynaptic terminals with FM4-64, the appearance of 
      new presynaptic terminals was clearly observed on newly formed filopodia after 
      120 min treatment with bicuculline. These results suggest that 
      bicuculline-induced [Ca2+]in oscillation of MT cells induces the growth of 
      filopodia and subsequently the formation of new presynaptic terminals. 
      Furthermore, tetrodotoxin or the deprivation of extracellular calcium blocked 
      bicuculline-induced synapse formation. The present results indicate that the 
      long-lasting [Ca2+]in oscillation caused by bicuculline-induced disinhibition of 
      cultured MT cells is significantly implicated in the mechanism underlying synapse 
      formation on cultured AOB neurons. Our established culture system of AOB neurons 
      will aid in clarifying the mechanism of synapse formation between AOB neurons and 
      the molecular mechanism of pheromonal memory formation.
FAU - Kato-Negishi, Midori
AU  - Kato-Negishi M
AD  - Laboratory of Anatomy and Cell Biology, Department of Basic Technique and 
      Facilities, Tokyo Metropolitan Institute for Neuroscience, Tokyo Metropolitan 
      Organization for Medical Research, 2-6 Musashi-dai Fuchu, Tokyo 183-8526, Japan. 
      midori@tmin.ac.jp
FAU - Muramoto, Kazuyo
AU  - Muramoto K
FAU - Kawahara, Masahiro
AU  - Kawahara M
FAU - Hosoda, Ritsuko
AU  - Hosoda R
FAU - Kuroda, Yoichiro
AU  - Kuroda Y
FAU - Ichikawa, Masumi
AU  - Ichikawa M
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - France
TA  - Eur J Neurosci
JT  - The European journal of neuroscience
JID - 8918110
RN  - 0 (Actins)
RN  - 0 (Anesthetics, Local)
RN  - 0 (FM 4-64)
RN  - 0 (GABA Antagonists)
RN  - 0 (Luminescent Proteins)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (Pyridinium Compounds)
RN  - 0 (Quaternary Ammonium Compounds)
RN  - 0 (Receptors, GABA-A)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 0 (Synapsins)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - 4368-28-9 (Tetrodotoxin)
RN  - 660YQ98I10 (Potassium Chloride)
RN  - EC 4.1.1.15 (Glutamate Decarboxylase)
RN  - SY7Q814VUP (Calcium)
RN  - TSN3DL106G (Fura-2)
RN  - Y37615DVKC (Bicuculline)
SB  - IM
MH  - Actins/metabolism
MH  - Anesthetics, Local/pharmacology
MH  - Animals
MH  - Animals, Newborn
MH  - Bicuculline/*pharmacology
MH  - Calcium/metabolism
MH  - Calcium Signaling
MH  - Cells, Cultured
MH  - Dendrites/drug effects/metabolism
MH  - Fura-2/metabolism
MH  - GABA Antagonists/*pharmacology
MH  - Glutamate Decarboxylase/metabolism
MH  - Green Fluorescent Proteins
MH  - Immunohistochemistry/methods
MH  - Luminescent Proteins/metabolism
MH  - Microscopy, Confocal/instrumentation/methods
MH  - Microtubule-Associated Proteins/metabolism
MH  - Neurons/classification/*drug effects/physiology
MH  - Olfactory Bulb/cytology/*drug effects/physiology
MH  - Potassium Chloride/pharmacology
MH  - Pseudopodia/drug effects/metabolism
MH  - Pyridinium Compounds/metabolism
MH  - Quaternary Ammonium Compounds/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Receptors, GABA-A/metabolism
MH  - Receptors, N-Methyl-D-Aspartate/metabolism
MH  - Synapses/classification/*drug effects/physiology
MH  - Synapsins/metabolism
MH  - Tetrodotoxin/pharmacology
MH  - Time Factors
MH  - Transfection
EDAT- 2003/09/27 05:00
MHDA- 2003/12/06 05:00
CRDT- 2003/09/27 05:00
PHST- 2003/09/27 05:00 [pubmed]
PHST- 2003/12/06 05:00 [medline]
PHST- 2003/09/27 05:00 [entrez]
AID - 2901 [pii]
AID - 10.1046/j.1460-9568.2003.02901.x [doi]
PST - ppublish
SO  - Eur J Neurosci. 2003 Sep;18(6):1343-52. doi: 10.1046/j.1460-9568.2003.02901.x.