PMID- 14511907
OWN - NLM
STAT- MEDLINE
DCOM- 20040609
LR  - 20190916
IS  - 0168-1656 (Print)
IS  - 0168-1656 (Linking)
VI  - 105
IP  - 1-2
DP  - 2003 Oct 9
TI  - In situ PCR for visualizing distribution of a functional gene "amoA" in a biofilm 
      regardless of activity.
PG  - 33-40
AB  - In this study, ammonia-oxidizing bacteria present in biofilms resulting from a 
      nitrifying reactor were detected by both a conventional FISH technique and an 
      original in situ PCR technique. Both techniques showed that ammonia-oxidizing 
      bacteria were found near the surface of the biofilms. However, after the biofilm 
      had been exposed to 2 weeks of ammonia starvation, ammonia-oxidizing bacteria 
      present in the biofilm could not be detected by fluorescence in situ 
      hybridization (FISH) because they did not have sufficient copies of rRNA. In 
      contrast, ammonia-oxidizing bacteria could be detected by in situ PCR with strong 
      signal. It was thus demonstrated that a cell possessing a specific functional 
      gene is detectable by in situ PCR regardless of its activity.
FAU - Hoshino, Tatsuhiko
AU  - Hoshino T
AD  - Department of Chemical Engineering, Waseda University, 3-4-1 Ohkubo, Shinjuku-ku, 
      Tokyo 169-8555, Japan. t-hoshino@ruri.waseda.jp
FAU - Tsuneda, Satoshi
AU  - Tsuneda S
FAU - Hirata, Akira
AU  - Hirata A
FAU - Inamori, Yuhei
AU  - Inamori Y
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - J Biotechnol
JT  - Journal of biotechnology
JID - 8411927
RN  - 0 (DNA, Bacterial)
RN  - 0 (Oligonucleotides)
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 7664-41-7 (Ammonia)
RN  - EC 1.- (Oxidoreductases)
RN  - EC 1.7.3.- (ammonia monooxygenase)
SB  - IM
MH  - Ammonia/*metabolism
MH  - *Biofilms
MH  - Bioreactors
MH  - DNA, Bacterial/genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Oligonucleotides/genetics
MH  - Oxidoreductases/genetics
MH  - Polymerase Chain Reaction/*methods
MH  - RNA, Bacterial/genetics
MH  - RNA, Ribosomal, 16S/genetics
EDAT- 2003/09/27 05:00
MHDA- 2004/06/21 10:00
CRDT- 2003/09/27 05:00
PHST- 2003/09/27 05:00 [pubmed]
PHST- 2004/06/21 10:00 [medline]
PHST- 2003/09/27 05:00 [entrez]
AID - S0168165603001718 [pii]
AID - 10.1016/s0168-1656(03)00171-8 [doi]
PST - ppublish
SO  - J Biotechnol. 2003 Oct 9;105(1-2):33-40. doi: 10.1016/s0168-1656(03)00171-8.