PMID- 14512080
OWN - NLM
STAT- MEDLINE
DCOM- 20040525
LR  - 20190727
IS  - 0049-3848 (Print)
IS  - 0049-3848 (Linking)
VI  - 110
IP  - 4
DP  - 2003 Jun 1
TI  - Activated platelets trigger an inflammatory response and enhance migration of 
      aortic smooth muscle cells.
PG  - 187-94
AB  - OBJECTIVE: Exposure of the subendothelium to flowing blood following rupture of 
      atherosclerotic lesions or during balloon angioplasty initiates platelet adhesion 
      to the vascular wall. Because activated platelets release proinflammatory 
      mediators (e.g., interleukin (IL)-1beta) and secrete growth factors, platelet 
      adhesion to the subendothelial matrix might contribute to the recruitment of 
      inflammatory cells and promote migration and proliferation of vascular smooth 
      muscle cells (SMCs). METHODS AND RESULTS: Here, we demonstrate that incubation of 
      cultured monolayers of aortic SMCs with alpha-thrombin-activated platelets 
      significantly enhances the secretion of monocyte chemoattractant protein-1 
      (MCP-1) (P<0.05) and promotes SMC migration (P<0.05). Platelet-induced secretion 
      of MCP-1 was abolished by anti-IL-1alpha and beta monoclonal antibodies or the 
      IL-1 receptor antagonist (IL-1RA). In contrast, platelet-mediated SMC migration 
      was attenuated only by anti-platelet-derived growth factor (PDGF)-mAb but not by 
      IL-1RA. Correspondingly, recombinant human interleukin-1 (rhIL-1) beta increased 
      MCP release by SMCs but had no effect on SMC migration. Platelet-mediated MCP 
      secretion by SMCs involved the activation and nuclear translocation of the 
      transcription factor nuclear factor-kappaB (NF-kappaB). CONCLUSION: Therefore, 
      platelet adhesion to the subendothelium increases the chemotactic and migratory 
      properties of SMC and is likely to contribute substantially to the process of 
      atherosclerosis and vessel (re-)stenosis.
FAU - Massberg, Steffen
AU  - Massberg S
AD  - Medizinische Klinik and Deutsches Herzzentrum, Technische Universitat Munchen, 
      Lazarettstrasse 36, 80636 Munich, Germany. massberg@dhm.mhn.de
FAU - Vogt, Felix
AU  - Vogt F
FAU - Dickfeld, Timm
AU  - Dickfeld T
FAU - Brand, Korbinian
AU  - Brand K
FAU - Page, Sharon
AU  - Page S
FAU - Gawaz, Meinrad
AU  - Gawaz M
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Thromb Res
JT  - Thrombosis research
JID - 0326377
RN  - 0 (Chemokine CCL2)
SB  - IM
MH  - *Aorta/cytology
MH  - Blood Platelets/*metabolism
MH  - Cell Adhesion/physiology
MH  - Cells, Cultured
MH  - Chemokine CCL2/*metabolism
MH  - Humans
MH  - Inflammation
MH  - Muscle, Smooth, Vascular/*metabolism
MH  - *Platelet Activation
EDAT- 2003/09/27 05:00
MHDA- 2004/05/27 05:00
CRDT- 2003/09/27 05:00
PHST- 2003/09/27 05:00 [pubmed]
PHST- 2004/05/27 05:00 [medline]
PHST- 2003/09/27 05:00 [entrez]
AID - S0049384803003426 [pii]
AID - 10.1016/s0049-3848(03)00342-6 [doi]
PST - ppublish
SO  - Thromb Res. 2003 Jun 1;110(4):187-94. doi: 10.1016/s0049-3848(03)00342-6.