PMID- 14512438
OWN - NLM
STAT- MEDLINE
DCOM- 20040123
LR  - 20131121
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 145
IP  - 1
DP  - 2004 Jan
TI  - Brain-derived neurotrophic factor and androgen interact in the maintenance of 
      dendritic morphology in a sexually dimorphic rat spinal nucleus.
PG  - 161-8
AB  - Testosterone regulates androgen receptor expression, soma size, and dendritic 
      length in motoneurons of the spinal nucleus of the bulbocavernosus (SNB) in adult 
      male rats. Brain-derived neurotrophic factor (BDNF) is also expressed in SNB 
      motoneurons; BDNF maintains SNB soma size in castrates, and interacts with 
      testosterone to regulate androgen receptor expression in SNB motoneurons. This 
      study tested the hypotheses that BDNF promotes SNB dendritic lengths and that 
      BDNF and testosterone interact to maintain dendritic morphology in SNB 
      motoneurons. Adult male rats were castrated; and, 5 wk later, SNB motoneurons 
      were axotomized bilaterally, and BDNF or PBS was applied via cups sutured to the 
      cut axons. After axotomy plus BDNF or PBS application, castrates received 
      implants of testosterone or blank capsules and were killed 24 d later. Additional 
      males of similar age that received sham castration, sham axotomy, and a blank 
      implant served as sham controls. Two days before death, SNB motoneurons were 
      retrogradely labeled with cholera toxin-horseradish peroxidase, and SNB dendritic 
      morphology was reconstructed in three dimensions. Dendritic lengths in 
      blank-implanted castrates treated with PBS were significantly shorter than those 
      of sham controls; treatment with either testosterone or BDNF alone failed to 
      support dendritic length or distribution. However, treatment with both 
      testosterone and BDNF restored dendritic morphology to the level of sham 
      controls. Our findings demonstrate that BDNF interacts with testosterone in the 
      maintenance of SNB dendritic arbors and support the hypothesis that dendritic 
      morphology is regulated by trophic substances that originate in the neuromuscular 
      periphery.
FAU - Yang, L Y
AU  - Yang LY
AD  - Department of Physiology, Taipei Medical University, Taipei 110, Taiwan. 
      yangly@tmu.edu.tw
FAU - Verhovshek, T
AU  - Verhovshek T
FAU - Sengelaub, D R
AU  - Sengelaub DR
LA  - eng
GR  - HD35315/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20030925
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (Androgens)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 3XMK78S47O (Testosterone)
SB  - IM
MH  - Androgens/*pharmacology
MH  - Animals
MH  - Axotomy
MH  - Brain-Derived Neurotrophic Factor/*pharmacology
MH  - Dendrites/*drug effects/physiology
MH  - Drug Interactions
MH  - Male
MH  - Motor Neurons/drug effects/ultrastructure
MH  - Orchiectomy
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - *Sex Characteristics
MH  - Spinal Cord/*cytology
MH  - Testosterone/*pharmacology
EDAT- 2003/09/27 05:00
MHDA- 2004/01/24 05:00
CRDT- 2003/09/27 05:00
PHST- 2003/09/27 05:00 [pubmed]
PHST- 2004/01/24 05:00 [medline]
PHST- 2003/09/27 05:00 [entrez]
AID - en.2003-0853 [pii]
AID - 10.1210/en.2003-0853 [doi]
PST - ppublish
SO  - Endocrinology. 2004 Jan;145(1):161-8. doi: 10.1210/en.2003-0853. Epub 2003 Sep 
      25.