PMID- 14512886
OWN - NLM
STAT- MEDLINE
DCOM- 20031030
LR  - 20061115
IS  - 0270-9139 (Print)
IS  - 0270-9139 (Linking)
VI  - 38
IP  - 4
DP  - 2003 Oct
TI  - High frequency of chimerism in transplanted livers.
PG  - 989-98
AB  - Recent studies have shown that primitive stem cells can mobilize and 
      differentiate into hepatocytes. We investigated the time and extent in which 
      cells of recipient origins could differentiate into hepatocytes and other cells 
      in human liver allografts. Microsatellite analysis, which can assess 
      quantitatively the proportions of recipient and donor DNA, was performed in 
      posttransplantation liver biopsy specimens from 17 patients at various times. 
      Combined fluorescence in situ hybridization (FISH) for Y chromosome and 
      immunofluorescence for different cell types was also performed in 10 of these 
      cases with sex mismatch. Organ chimerism in the transplanted livers was found to 
      be of variable extent, and the recipients' DNA in the posttransplantation liver 
      biopsy specimens (excluding portal tracts) amounted up to 50%. The recipient DNA 
      in the posttransplantation liver biopsy specimens increased after liver 
      transplantation by as early as 1 week, peaked at around 30 to 40 weeks, and could 
      be shown 63 weeks after transplantation. Most (64%-75%) of the recipient-derived 
      cells showed macrophage/Kupffer cell differentiation. Only up to 1.6% of the 
      recipient-derived cells in the liver grafts showed hepatocytic differentiation in 
      the liver grafts and made up 0.62% of all hepatocytes of both donor and recipient 
      origins. These livers had mild or minimal injury histologically. In conclusion, 
      our results show that most of the recipient-derived cells in the liver allografts 
      were macrophages/Kupffer cells and only a small proportion of hepatocytes was 
      recipient derived. However, with regard to recipient-derived hepatocytes, our 
      data cannot distinguish between transdifferentiation and cell fusion.
FAU - Ng, Irene Oi-Lin
AU  - Ng IO
AD  - Department of Pathology, Faculty of Medicine, University of Hong Kong, Queen Mary 
      Hospital, 102 Pokfulam Road, Hong Kong. iolng@hku.hk
FAU - Chan, Kok-Lung
AU  - Chan KL
FAU - Shek, Wai-Hung
AU  - Shek WH
FAU - Lee, Joyce Man-Fong
AU  - Lee JM
FAU - Fong, Daniel Yee-Tak
AU  - Fong DY
FAU - Lo, Chung-Mau
AU  - Lo CM
FAU - Fan, Sheung-Tat
AU  - Fan ST
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Hepatology
JT  - Hepatology (Baltimore, Md.)
JID - 8302946
SB  - IM
CIN - Hepatology. 2003 Oct;38(4):804-6. PMID: 14512866
CIN - Gastroenterology. 2004 Jul;127(1):346-8. PMID: 15236208
MH  - Adult
MH  - Cell Differentiation
MH  - Chromosomes, Human, Y
MH  - Female
MH  - Fluorescent Antibody Technique
MH  - Hepatocytes/*cytology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Kupffer Cells/cytology
MH  - *Liver Transplantation
MH  - Macrophages/cytology
MH  - Male
MH  - Middle Aged
MH  - *Transplantation Chimera
MH  - Transplantation, Homologous
EDAT- 2003/09/27 05:00
MHDA- 2003/10/31 05:00
CRDT- 2003/09/27 05:00
PHST- 2003/09/27 05:00 [pubmed]
PHST- 2003/10/31 05:00 [medline]
PHST- 2003/09/27 05:00 [entrez]
AID - S0270913903007006 [pii]
AID - 10.1053/jhep.2003.50395 [doi]
PST - ppublish
SO  - Hepatology. 2003 Oct;38(4):989-98. doi: 10.1053/jhep.2003.50395.