PMID- 1451456
OWN - NLM
STAT- MEDLINE
DCOM- 19930107
LR  - 20190721
IS  - 0105-1873 (Print)
IS  - 0105-1873 (Linking)
VI  - 27
IP  - 3
DP  - 1992 Sep
TI  - The value of the local lymph node assay in quantitative structure-activity 
      investigations.
PG  - 137-42
AB  - The development of quantitative correlations between the physicochemical 
      properties of a compound and its ability to act as a skin sensitizer is 
      complicated by the number of variables associated with the current sensitization 
      test data, combined with the absence of a truly objective end point. Recently, 
      however, a novel approach to the assessment of skin sensitization potential, the 
      local lymph node assay (LLNA), has been described, which determines the skin 
      sensitization by measuring lymphocyte proliferation in lymph nodes draining the 
      site of chemical exposure. The assay offers several advantages over traditional 
      methods in the context of quantitative structure-activity relationship studies. 
      In the present work, a range of bromoalkanes has been employed which demonstrate 
      the robustness and reproducibility of the LLNA. Sensitizing activity increased 
      with chain length up to a maximum at C15/C16, whereafter the response declined. 
      The data were modelled against hydrophobicity, expressed as Clog P and (ClogP)2 
      to fit the biphasic nature of the results. The results demonstrate the utility of 
      LLNA data for interpretation in the context of quantitative structure-activity 
      relationships, the limited number of variables, inter-test reproducibility and 
      quantitative end point, lending themselves to mathematical interpretations.
FAU - Basketter, D A
AU  - Basketter DA
AD  - Unilever Environmental Safety Laboratory, Sharnbrook, Beds, UK.
FAU - Roberts, D W
AU  - Roberts DW
FAU - Cronin, M
AU  - Cronin M
FAU - Scholes, E W
AU  - Scholes EW
LA  - eng
PT  - Journal Article
PL  - England
TA  - Contact Dermatitis
JT  - Contact dermatitis
JID - 7604950
RN  - 0 (Hydrocarbons, Brominated)
SB  - IM
MH  - Animals
MH  - Dermatitis, Contact/*diagnosis/etiology
MH  - Dose-Response Relationship, Drug
MH  - Hydrocarbons, Brominated/*adverse effects
MH  - Lymph Nodes/*immunology
MH  - Lymphocyte Activation/*drug effects
MH  - Mice
MH  - Mice, Inbred CBA
MH  - Reproducibility of Results
MH  - Structure-Activity Relationship
EDAT- 1992/09/01 00:00
MHDA- 1992/09/01 00:01
CRDT- 1992/09/01 00:00
PHST- 1992/09/01 00:00 [pubmed]
PHST- 1992/09/01 00:01 [medline]
PHST- 1992/09/01 00:00 [entrez]
AID - 10.1111/j.1600-0536.1992.tb05241.x [doi]
PST - ppublish
SO  - Contact Dermatitis. 1992 Sep;27(3):137-42. doi: 
      10.1111/j.1600-0536.1992.tb05241.x.