PMID- 14515192
OWN - NLM
STAT- MEDLINE
DCOM- 20040706
LR  - 20091119
IS  - 0340-6245 (Print)
IS  - 0340-6245 (Linking)
VI  - 90
IP  - 4
DP  - 2003 Oct
TI  - Evidence for functionally active protease-activated receptor-3 (PAR-3) in human 
      vascular smooth muscle cells.
PG  - 704-9
AB  - The present study investigates whether vascular smooth muscle cells of the human 
      saphenous vein (SMC) express a functionally active protease-activated receptor-3 
      (PAR-3). PAR-3 mRNA was detected by RT-PCR. In the presence of thrombin, a rapid 
      and transient increase in PAR-3 mRNA was observed. Stimulation of SMC with 
      thrombin or the synthetic PAR-3-activating peptide, TFRGAP, resulted in transient 
      mobilization of intracellular calcium. After a preceding challenge with thrombin, 
      the calcium signal to TFRGAP was abolished, suggesting cleavage and subsequent 
      desensitization of PAR-3 by thrombin. Activation of PAR-3 by TFRGAP elicited a 
      time-dependent activation of the extracellular-signal-regulated kinase (ERK)-1/2 
      with a maximum response 10-20 min after stimulation. At 200 microM, TFRGAP 
      increased [3H]-thymidine incorporation into cellular DNA about two-fold. These 
      data indicate that PAR-3 is expressed in human SMC and triggers intracellular 
      signaling. Thus, in the SMC PAR-3 might contribute to thrombin-induced responses.
FAU - Bretschneider, Ellen
AU  - Bretschneider E
AD  - Institut fur Vaskulare Medizin Erfurt, Friedrich-Schiller-Universitat Jena, 
      Erfurt, Germany. bretschn@zmkh.ef.uni-jena.de
FAU - Spanbroek, Rainer
AU  - Spanbroek R
FAU - Lotzer, Katharina
AU  - Lotzer K
FAU - Habenicht, Andreas Johann Richard
AU  - Habenicht AJ
FAU - Schror, Karsten
AU  - Schror K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Thromb Haemost
JT  - Thrombosis and haemostasis
JID - 7608063
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Thrombin)
RN  - 0 (protease-activated receptor 3)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 3.4.21.5 (Thrombin)
SB  - IM
MH  - Calcium Signaling
MH  - DNA Replication/drug effects
MH  - Humans
MH  - Kinetics
MH  - Mitogen-Activated Protein Kinase 3
MH  - Mitogen-Activated Protein Kinases/metabolism
MH  - Muscle, Smooth, Vascular/chemistry/*cytology
MH  - Myocytes, Smooth Muscle/*chemistry
MH  - RNA, Messenger/analysis/drug effects
MH  - Receptors, Thrombin/*analysis/genetics/physiology
MH  - Saphenous Vein
MH  - Thrombin/pharmacology
EDAT- 2003/09/30 05:00
MHDA- 2004/07/09 05:00
CRDT- 2003/09/30 05:00
PHST- 2003/09/30 05:00 [pubmed]
PHST- 2004/07/09 05:00 [medline]
PHST- 2003/09/30 05:00 [entrez]
AID - 03100704 [pii]
AID - 10.1160/TH03-04-0203 [doi]
PST - ppublish
SO  - Thromb Haemost. 2003 Oct;90(4):704-9. doi: 10.1160/TH03-04-0203.