PMID- 14515469
OWN - NLM
STAT- MEDLINE
DCOM- 20031120
LR  - 20071115
IS  - 0016-6758 (Print)
IS  - 0016-6758 (Linking)
VI  - 39
IP  - 8
DP  - 2003 Aug
TI  - [Molecular cytogenetic characteristics of chromosome imbalance in cells of 
      spontaneous human abortion fetuses with low proliferative activity in vitro].
PG  - 1111-22
AB  - Karyotyping of noncultivated cells of 60 first-trimester spontaneous abortions 
      (blighted ova and missed abortions) was carried out using fluorescence in situ 
      hybridization (FISH) with centromere-specific DNA probes for all chromosomes of 
      the karyotype. Conventional cytogenetic study of these abortions was impossible 
      because of cell culture failures. The algorithm is proposed for molecular 
      cytogenetic FISH analysis of interphase karyotypes. Chromosome abnormalities were 
      found in 32 fetuses (53.3%). In groups of missed abortions and blighted ova, the 
      frequency of numerical chromosome abnormalities was 50 and 60%, respectively. 
      Both the numerical chromosome abnormalities typical of spontaneous human 
      abortions (autosomal trisomies, sex chromosome aneuploidy, and polyploidy) and a 
      relatively rare type of genomic imbalance unidentifiable by standard cytogenetic 
      analysis (autosomal monosomies 7, 15, 21, and 22 in mosaic state) were observed. 
      The frequency of these type of chromosome abnormalities comprised 19% of all 
      known karyotype abnormalities determined in spontaneously perished embryos. Note 
      that the level of confined placental mosaicism in embryos with low cell 
      proliferative activity was 25%, which is substantially higher than the 
      corresponding parameter (1-2%) determined by prenatal diagnosis of chromosome 
      abnormalities in developing embryos. The results of interphase FISH analysis of 
      cells with low proliferative activity in vitro suggest that the pathology of 
      early fetal development and missed abortion in humans are associated with a wider 
      spectrum of chromosome abnormalities.
FAU - Lebedev, I N
AU  - Lebedev IN
AD  - Research Institute of Medical Genetics, Tomsk Research Center, Russian Academy of 
      Medical Sciences, Tomsk, 634050 Russia. igorl@img.tsu.ru
FAU - Ostroverkhova, N V
AU  - Ostroverkhova NV
FAU - Nikitina, T V
AU  - Nikitina TV
FAU - Sukhanova, N N
AU  - Sukhanova NN
FAU - Nazarenko, S A
AU  - Nazarenko SA
LA  - rus
PT  - English Abstract
PT  - Journal Article
TT  - Molekuliarno-tsitogeneticheskaia kharakteristika khromosomnogo disbalansa v 
      kletkakh spontannykh abortusov cheloveka s nizkoi proliferativnoi aktivnost'iu in 
      vitro.
PL  - Russia (Federation)
TA  - Genetika
JT  - Genetika
JID - 0047354
SB  - IM
MH  - Abortion, Spontaneous
MH  - Cell Division/physiology
MH  - Cells, Cultured
MH  - Chromosome Aberrations
MH  - *Cytogenetic Analysis
MH  - Embryo, Mammalian/*cytology/*physiology
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Placenta/cytology
MH  - Pregnancy
EDAT- 2003/10/01 05:00
MHDA- 2003/12/03 05:00
CRDT- 2003/10/01 05:00
PHST- 2003/10/01 05:00 [pubmed]
PHST- 2003/12/03 05:00 [medline]
PHST- 2003/10/01 05:00 [entrez]
PST - ppublish
SO  - Genetika. 2003 Aug;39(8):1111-22.