PMID- 14517096
OWN - NLM
STAT- MEDLINE
DCOM- 20031103
LR  - 20190714
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 314
IP  - 1
DP  - 2003 Sep 15
TI  - Structure of replicating intermediates of human herpesvirus type 6.
PG  - 443-50
AB  - We have studied the structure of the replicative intermediates of human 
      herpesvirus 6 (HHV-6) using pulsed-field gel electrophoresis, partial digestion, 
      two-dimensional gel electrophoresis, and sedimentation centrifugation. The 
      results show that DNA replication of HHV-6 produces head-to-tail concatemeric 
      intermediates as well as approximately equal amounts of circular monomers or 
      oligomers. Unlike the situation in herpes simplex virus, the intermediates of 
      human herpesvirus 6 replication are not highly branched, suggesting a difference 
      in the mechanism of replication or a lower frequency of homologous recombination 
      in human herpesvirus 6 compared to herpes simplex virus.
FAU - Severini, Alberto
AU  - Severini A
AD  - National Microbiology Laboratory, Health Canada, Winnipeg, Monitoba, Canada. 
      Alberto_Severini@hc-sc.gc.ca
FAU - Sevenhuysen, Claire
AU  - Sevenhuysen C
FAU - Garbutt, Michael
AU  - Garbutt M
FAU - Tipples, Graham A
AU  - Tipples GA
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (DNA, Viral)
RN  - EC 3.1.21.- (endodeoxyribonuclease SfiI)
RN  - EC 3.1.21.4 (Deoxyribonucleases, Type II Site-Specific)
SB  - IM
MH  - Cell Line
MH  - Centrifugation, Density Gradient
MH  - *DNA Replication
MH  - DNA, Viral/*biosynthesis/*chemistry/genetics
MH  - Deoxyribonucleases, Type II Site-Specific/metabolism
MH  - Electrophoresis, Gel, Pulsed-Field
MH  - Electrophoresis, Gel, Two-Dimensional
MH  - Herpesvirus 6, Human/*genetics/metabolism/physiology
MH  - Humans
EDAT- 2003/10/01 05:00
MHDA- 2003/11/05 05:00
CRDT- 2003/10/01 05:00
PHST- 2003/10/01 05:00 [pubmed]
PHST- 2003/11/05 05:00 [medline]
PHST- 2003/10/01 05:00 [entrez]
AID - S0042682203004513 [pii]
AID - 10.1016/s0042-6822(03)00451-3 [doi]
PST - ppublish
SO  - Virology. 2003 Sep 15;314(1):443-50. doi: 10.1016/s0042-6822(03)00451-3.