PMID- 14517466
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20191120
IS  - 1573-7209 (Electronic)
IS  - 0969-6970 (Linking)
VI  - 2
IP  - 3
DP  - 1998
TI  - Angiogenesis induced by tumor necrosis factor-agr; is mediated by alpha4 
      integrins.
PG  - 265-75
AB  - Tumor necrosis factor-alpha (TNF-alpha) and fibroblast growth factor-2 (FGF-2 or 
      bFGF) are potent stimulators of angiogenesis. TNF- alpha, but not FGF-2, can 
      induce the expression of vascular cell adhesion molecule-1 (VCAM-1) on the 
      surface of endothelial cells. The soluble form of VCAM-1 has recently been 
      demonstrated to function as an angiogenic mediator. Here we demonstrate that 
      monoclonal antibodies directed against VCAM-1 or its alpha4 integrin counter- 
      receptor inhibited TNF-alpha-induced endothelial cell migration in vitro. 
      Angiogenesis induced in vivo in rat corneas by TNF-alpha was inhibited by a 
      neutralizing antibody directed against the rat alpha4 integrin subunit. A peptide 
      antagonist of the a4 integrins blocked TNF-alpha-induced endothelial cell 
      migration in vitro and angiogenesis in rat corneas in vivo. No inhibition by the 
      antibodies or peptide antagonist was observed either in vitro or in vivo when 
      FGF-2 was used as the stimulus. The peptide antagonist did not inhibit TNF-a 
      binding to its receptor nor did it block the function of alphavbeta3, an integrin 
      previously implicated in TNF-a and FGF- 2 mediated angiogenesis. These results 
      demonstrate that angiogenic processes induced by TNF-alpha are mediated in part 
      by agr;4 integrins possibly by a mechanism involving the induction of soluble 
      VCAM-1.
FAU - Vanderslice, P
AU  - Vanderslice P
AD  - Department of Immunology, Texas Biotechnology Corporation, Houston, TX, USA.
FAU - Munsch, C L
AU  - Munsch CL
FAU - Rachal, E
AU  - Rachal E
FAU - Erichsen, D
AU  - Erichsen D
FAU - Sughrue, K M
AU  - Sughrue KM
FAU - Truong, A N
AU  - Truong AN
FAU - Wygant, J N
AU  - Wygant JN
FAU - McIntyre, B W
AU  - McIntyre BW
FAU - Eskin, S G
AU  - Eskin SG
FAU - Tilton, R G
AU  - Tilton RG
FAU - Polverini, P J
AU  - Polverini PJ
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Angiogenesis
JT  - Angiogenesis
JID - 9814575
EDAT- 2003/10/01 05:00
MHDA- 2003/10/01 05:01
CRDT- 2003/10/01 05:00
PHST- 2003/10/01 05:00 [pubmed]
PHST- 2003/10/01 05:01 [medline]
PHST- 2003/10/01 05:00 [entrez]
AID - 188219 [pii]
AID - 10.1023/a:1009296700991 [doi]
PST - ppublish
SO  - Angiogenesis. 1998;2(3):265-75. doi: 10.1023/a:1009296700991.