PMID- 14519200
OWN - NLM
STAT- MEDLINE
DCOM- 20040810
LR  - 20181113
IS  - 1474-760X (Electronic)
IS  - 1465-6906 (Print)
IS  - 1474-7596 (Linking)
VI  - 4
IP  - 10
DP  - 2003
TI  - Annotation and analysis of 10,000 expressed sequence tags from developing mouse 
      eye and adult retina.
PG  - R65
AB  - BACKGROUND: As a biomarker of cellular activities, the transcriptome of a 
      specific tissue or cell type during development and disease is of great 
      biomedical interest. We have generated and analyzed 10,000 expressed sequence 
      tags (ESTs) from three mouse eye tissue cDNA libraries: embryonic day 15.5 (M15E) 
      eye, postnatal day 2 (M2PN) eye and adult retina (MRA). RESULTS: Annotation of 
      8,633 non-mitochondrial and non-ribosomal high-quality ESTs revealed that 57% of 
      the sequences represent known genes and 43% are unknown or novel ESTs, with M15E 
      having the highest percentage of novel ESTs. Of these, 2,361 ESTs correspond to 
      747 unique genes and the remaining 6,272 are represented only once. 
      Phototransduction genes are preferentially identified in MRA, whereas transcripts 
      for cell structure and regulatory proteins are highly expressed in the developing 
      eye. Map locations of human orthologs of known genes uncovered a high density of 
      ocular genes on chromosome 17, and identified 277 genes in the critical regions 
      of 37 retinal disease loci. In silico expression profiling identified 210 genes 
      and/or ESTs over-expressed in the eye; of these, more than 26 are known to have 
      vital retinal function. Comparisons between libraries provided a list of 
      temporally regulated genes and/or ESTs. A few of these were validated by qRT-PCR 
      analysis. CONCLUSIONS: Our studies present a large number of potentially 
      interesting genes for biological investigation, and the annotated EST set 
      provides a useful resource for microarray and functional genomic studies.
FAU - Yu, Jindan
AU  - Yu J
AD  - Ophthalmology and Visual Science, University of Michigan, 1000 Wall Street, Ann 
      Arbor, MI 48105, USA.
FAU - Farjo, Rafal
AU  - Farjo R
FAU - MacNee, Sean P
AU  - MacNee SP
FAU - Baehr, Wolfgang
AU  - Baehr W
FAU - Stambolian, Dwight E
AU  - Stambolian DE
FAU - Swaroop, Anand
AU  - Swaroop A
LA  - eng
GR  - EY11115/EY/NEI NIH HHS/United States
GR  - EY08123/EY/NEI NIH HHS/United States
GR  - R01 EY011115/EY/NEI NIH HHS/United States
GR  - EY10321/EY/NEI NIH HHS/United States
GR  - R01 EY007961/EY/NEI NIH HHS/United States
GR  - EY07003/EY/NEI NIH HHS/United States
GR  - EY07961/EY/NEI NIH HHS/United States
GR  - R01 EY008123/EY/NEI NIH HHS/United States
GR  - P30 EY007003/EY/NEI NIH HHS/United States
GR  - F31 EY007003/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20030922
PL  - England
TA  - Genome Biol
JT  - Genome biology
JID - 100960660
RN  - 0 (DNA, Complementary)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Aging/*genetics
MH  - Animals
MH  - Cluster Analysis
MH  - DNA, Complementary/genetics
MH  - *Expressed Sequence Tags
MH  - Eye/*growth & development/*metabolism
MH  - Eye Diseases/genetics
MH  - Gene Expression Profiling
MH  - Gene Library
MH  - Genomics
MH  - Humans
MH  - Mice
MH  - RNA, Messenger/analysis/genetics
MH  - Retina/*metabolism
MH  - Vision, Ocular/genetics
PMC - PMC328454
EDAT- 2003/10/02 05:00
MHDA- 2004/08/11 05:00
PMCR- 2003/09/22
CRDT- 2003/10/02 05:00
PHST- 2003/05/21 00:00 [received]
PHST- 2003/07/01 00:00 [revised]
PHST- 2003/08/19 00:00 [accepted]
PHST- 2003/10/02 05:00 [pubmed]
PHST- 2004/08/11 05:00 [medline]
PHST- 2003/10/02 05:00 [entrez]
PHST- 2003/09/22 00:00 [pmc-release]
AID - gb-2003-4-10-r65 [pii]
AID - 10.1186/gb-2003-4-10-r65 [doi]
PST - ppublish
SO  - Genome Biol. 2003;4(10):R65. doi: 10.1186/gb-2003-4-10-r65. Epub 2003 Sep 22.