PMID- 14522067
OWN - NLM
STAT- MEDLINE
DCOM- 20040219
LR  - 20191210
IS  - 1386-6532 (Print)
IS  - 1386-6532 (Linking)
VI  - 28
IP  - 3
DP  - 2003 Dec
TI  - Generation and characterization of a monoclonal antibody specific for human 
      herpesvirus 6 variant A immediate-early 2 protein.
PG  - 284-90
AB  - Two variants (A and B) of human herpesvirus 6 (HHV-6) can be isolated from 
      humans, with each variant having unique biological properties. HHV-6 variant 
      typing is mainly done following amplification of viral genomic DNA followed by 
      restriction endonuclease digestion. Our objective was to generate a monoclonal 
      antibody (mAb) that would allow us to discriminate between variants A and B of 
      HHV-6. BALB/c mice were immunized with a recombinant glutathione-S-transferase 
      protein fused to the immediate-early (IE) 2 protein from HHV-6 variant A. 
      Following splenocytes fusion, one IgG1 kappa light chain mAb (P6H8) was isolated 
      and found to react specifically with variant A IE2 protein in immunofluorescence 
      and western blot assays. The P6H8 antibody represents a useful tool for both 
      fundamental research and clinical applications allowing for the discrimination of 
      infections caused by HHV-6 variants A or B.
FAU - Arsenault, Suzie
AU  - Arsenault S
AD  - Virocell Inc, 2525 Laurier Blvd, Tour des Laurentides, Sainte-Foy, Quebec, Canada 
      GIV 2L2.
FAU - Gravel, Annie
AU  - Gravel A
FAU - Gosselin, Jean
AU  - Gosselin J
FAU - Flamand, Louis
AU  - Flamand L
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PL  - Netherlands
TA  - J Clin Virol
JT  - Journal of clinical virology : the official publication of the Pan American 
      Society for Clinical Virology
JID - 9815671
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antibodies, Viral)
RN  - 0 (Immediate-Early Proteins)
RN  - 0 (Recombinant Proteins)
RN  - EC 2.5.1.18 (Glutathione Transferase)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/immunology
MH  - Antibodies, Viral/immunology
MH  - Antibody Specificity
MH  - Cell Line
MH  - Flow Cytometry
MH  - *Genetic Variation
MH  - Glutathione Transferase/genetics/immunology/metabolism
MH  - Herpesvirus 6, Human/*classification/genetics
MH  - Humans
MH  - Immediate-Early Proteins/genetics/immunology/metabolism
MH  - Immunization
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Recombinant Proteins/genetics/immunology/metabolism
EDAT- 2003/10/03 05:00
MHDA- 2004/02/20 05:00
CRDT- 2003/10/03 05:00
PHST- 2003/10/03 05:00 [pubmed]
PHST- 2004/02/20 05:00 [medline]
PHST- 2003/10/03 05:00 [entrez]
AID - S1386653203000507 [pii]
AID - 10.1016/s1386-6532(03)00050-7 [doi]
PST - ppublish
SO  - J Clin Virol. 2003 Dec;28(3):284-90. doi: 10.1016/s1386-6532(03)00050-7.