PMID- 14522430
OWN - NLM
STAT- MEDLINE
DCOM- 20040224
LR  - 20190901
IS  - 0378-8741 (Print)
IS  - 0378-8741 (Linking)
VI  - 89
IP  - 1
DP  - 2003 Nov
TI  - Radical-scavenging effects of Aloe arborescens Miller on prevention of pancreatic 
      islet B-cell destruction in rats.
PG  - 37-45
AB  - We evaluated the possible scavenging effects of Aloe arborescens Miller var. 
      natalensis Berger (Kidachi aloe in Japanese) on free radicals generated by 
      streptozotocin (Sz) or alloxan (Ax). The components of Kidachi aloe were added to 
      a reaction system in which .OH radicals derived from Sz or Ax as pancreatic islet 
      B-cell toxins and hypoxanthine-xanthine oxidase (HX-XO)-derived O(2) radicals 
      destroy isolated islet B-cells, and we observed its preventive effects. The 
      Kidachi aloe components inhibited the destruction of rat pancreatic islet B-cells 
      by Sz, Ax or HX-XO. These components were prepared in the form of a freeze-dried 
      powder of the boiled leaf skin of Kidachi aloe, and measurement of 
      1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity showed higher 
      radical-scavenging activity in this boiled leaf skin powder than the non-boiled 
      leaf skin powder.Furthermore, HPLC chromatograms of the "Boiled leaf skin powder" 
      were similar to those of commercially available aloin (barbaloin content: 
      approximately 20%). Therefore, the main component may be a phenol compound. In 
      addition, the phenolic fraction of the Boiled leaf skin contained large amounts 
      of 2'-O-p-coumaroylaloesin and 2'-O-feruloylaloesin, which have higher DPPH 
      radical-scavenging activity than barbaloin. These results suggest that the action 
      mechanism of Kidachi aloe Boiled leaf skin components, which prevent destruction 
      of the pancreatic islets by specific pancreatic islet toxins such as Sz, Ax, and 
      HX-XO, involves inhibition of free radical-scavenging effects, and may be 
      associated with a thermostable low molecular component. The co-existence of 
      Kidachi aloe-derived 2'-O-p-coumaroylaloesin, 2'-O-feruloylaloesin, and aloin may 
      result in the potentiation of radical-scavenging activity.
FAU - Beppu, Hidehiko
AU  - Beppu H
AD  - Fujita Memorial Institute of Pharmacognosy, Fujita Health University, 1865 
      Isshiki-cho, Hisai, Mie 514-1296, Japan. hbeppu@fujita-hu.ac.jp
FAU - Koike, Takaaki
AU  - Koike T
FAU - Shimpo, Kan
AU  - Shimpo K
FAU - Chihara, Takeshi
AU  - Chihara T
FAU - Hoshino, Motoyuki
AU  - Hoshino M
FAU - Ida, Chikako
AU  - Ida C
FAU - Kuzuya, Hiroshi
AU  - Kuzuya H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - J Ethnopharmacol
JT  - Journal of ethnopharmacology
JID - 7903310
RN  - 0 (Antioxidants)
RN  - 0 (Free Radical Scavengers)
RN  - 0 (Plant Extracts)
RN  - 0 (Plant Preparations)
RN  - 0 (Reactive Oxygen Species)
RN  - 5W494URQ81 (Streptozocin)
RN  - 6SW5YHA5NG (Alloxan)
RN  - KA46RNI6HN (Emodin)
RN  - W41H6S09F4 (alloin)
SB  - IM
MH  - Alloxan/pharmacology
MH  - *Aloe
MH  - Animals
MH  - Antioxidants/*pharmacology
MH  - Cells, Cultured
MH  - Chromatography, High Pressure Liquid
MH  - Emodin/analogs & derivatives/isolation & purification/pharmacology
MH  - Free Radical Scavengers/*pharmacology
MH  - Islets of Langerhans/*drug effects/metabolism
MH  - Plant Extracts/pharmacology
MH  - Plant Leaves
MH  - Plant Preparations/*pharmacology
MH  - Rats
MH  - Rats, Wistar
MH  - Reactive Oxygen Species/*metabolism
MH  - Streptozocin/pharmacology
EDAT- 2003/10/03 05:00
MHDA- 2004/02/26 05:00
CRDT- 2003/10/03 05:00
PHST- 2003/10/03 05:00 [pubmed]
PHST- 2004/02/26 05:00 [medline]
PHST- 2003/10/03 05:00 [entrez]
AID - S037887410300268X [pii]
AID - 10.1016/s0378-8741(03)00268-x [doi]
PST - ppublish
SO  - J Ethnopharmacol. 2003 Nov;89(1):37-45. doi: 10.1016/s0378-8741(03)00268-x.