PMID- 14522917
OWN - NLM
STAT- MEDLINE
DCOM- 20031204
LR  - 20211203
IS  - 0008-5472 (Print)
IS  - 0008-5472 (Linking)
VI  - 63
IP  - 18
DP  - 2003 Sep 15
TI  - Mislocalization of membrane proteins associated with multidrug resistance in 
      cisplatin-resistant cancer cell lines.
PG  - 5909-16
AB  - The accumulation of [(14)C]carboplatin and [(3)H]methotrexate is reduced in 
      single-step KB epidermoid adenocarcinoma (KB-CP) cells, which are cross-resistant 
      to carboplatin, methotrexate, and sodium arsenite. In these KB-CP cells, 
      multidrug resistance is accompanied by mislocalization of multidrug resistance 
      associated protein (MRP) 1 and other membrane proteins such as folate-binding 
      protein. MRP1 was not decreased in amount in single-step variants but accumulates 
      in a cytoplasmic fraction, and its apparent molecular weight was altered probably 
      because of reduced glycosylation in resistant cells. This low-density compartment 
      was partially labeled with antibodies to lectin-GSII (a Golgi marker) and 
      Bip/GRP78 (an endoplasmic reticulum marker). Pulse-chase labeling of MRP1 with 
      (35)S-methionine and (35)S-cysteine and pulse-chase biotinylation of cell surface 
      MRP1 suggests that membrane protein mislocalization is caused mainly by a defect 
      of plasma membrane protein recycling, manifested also as a defect in 
      acidification of lysosomes. The reduced accumulation of cytotoxic compounds in 
      the KB-CP cells is presumed to result from the failure of carrier proteins and/or 
      transporters to localize to the plasma membrane.
FAU - Liang, Xing-Jie
AU  - Liang XJ
AD  - Laboratory of Cell Biology, Center for Cancer Research, National Cancer 
      Institute, National Institutes of Health, Bethesda, Maryland 20892-4254, USA.
FAU - Shen, Ding-Wu
AU  - Shen DW
FAU - Garfield, Susan
AU  - Garfield S
FAU - Gottesman, Michael M
AU  - Gottesman MM
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cancer Res
JT  - Cancer research
JID - 2984705R
RN  - 0 (ATP Binding Cassette Transporter, Subfamily B, Member 1)
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Carbon Radioisotopes)
RN  - 0 (Carrier Proteins)
RN  - 0 (Endoplasmic Reticulum Chaperone BiP)
RN  - 0 (Folate Receptors, GPI-Anchored)
RN  - 0 (HSPA5 protein, human)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Succinimides)
RN  - 0 (Sulfur Radioisotopes)
RN  - 0 (Triiodobenzoic Acids)
RN  - 0 (sulfo-N-hydroxysuccinimide-biotin)
RN  - 10028-17-8 (Tritium)
RN  - 6SO6U10H04 (Biotin)
RN  - AE28F7PNPL (Methionine)
RN  - BG3F62OND5 (Carboplatin)
RN  - HW8W27HTXX (iodixanol)
RN  - K848JZ4886 (Cysteine)
RN  - Q20Q21Q62J (Cisplatin)
RN  - YL5FZ2Y5U1 (Methotrexate)
SB  - IM
MH  - ATP Binding Cassette Transporter, Subfamily B, Member 1/*metabolism
MH  - Antineoplastic Agents/pharmacokinetics/*pharmacology
MH  - Biotin/*analogs & derivatives/metabolism
MH  - Carbon Radioisotopes
MH  - Carboplatin/pharmacokinetics
MH  - Carrier Proteins/*metabolism
MH  - Cell Membrane/metabolism
MH  - Cisplatin/pharmacokinetics/*pharmacology
MH  - Cysteine/metabolism
MH  - Drug Resistance, Multiple/*physiology
MH  - Drug Resistance, Neoplasm
MH  - Endoplasmic Reticulum Chaperone BiP
MH  - Folate Receptors, GPI-Anchored
MH  - HeLa Cells
MH  - Humans
MH  - KB Cells
MH  - Lysosomes/metabolism
MH  - Methionine/metabolism
MH  - Methotrexate/pharmacokinetics
MH  - *Receptors, Cell Surface
MH  - Succinimides/metabolism
MH  - Sulfur Radioisotopes
MH  - Triiodobenzoic Acids/chemistry
MH  - Tritium
EDAT- 2003/10/03 05:00
MHDA- 2003/12/05 05:00
CRDT- 2003/10/03 05:00
PHST- 2003/10/03 05:00 [pubmed]
PHST- 2003/12/05 05:00 [medline]
PHST- 2003/10/03 05:00 [entrez]
PST - ppublish
SO  - Cancer Res. 2003 Sep 15;63(18):5909-16.