PMID- 14528293
OWN - NLM
STAT- MEDLINE
DCOM- 20031224
LR  - 20220409
IS  - 1072-8368 (Print)
IS  - 1072-8368 (Linking)
VI  - 10
IP  - 11
DP  - 2003 Nov
TI  - The structure and binding mode of interleukin-18.
PG  - 966-71
AB  - Interleukin-18 (IL-18), a cytokine formerly known as interferon-gamma- 
      (IFN-gamma-) inducing factor, has pleiotropic immunoregulatory functions, 
      including augmentation of IFN-gamma production, Fas-mediated cytotoxicity and 
      developmental regulation of T-lymphocyte helper type I. We determined the 
      solution structure of IL-18 as a first step toward understanding its receptor 
      activation mechanism. It folds into a beta-trefoil structure that resembles that 
      of IL-1. Extensive mutagenesis revealed the presence of three sites that are 
      important for receptor activation: two serve as binding sites for IL-18 receptor 
      alpha (IL-18Ralpha), located at positions similar to those of IL-1 for IL-1 
      receptor type I (IL-1RI), whereas the third site may be involved in IL-18 
      receptor beta (IL-18Rbeta) binding. The structure and mutagenesis data provide a 
      basis for understanding the IL-18-induced heterodimerization of receptor 
      subunits, which is necessary for receptor activation.
FAU - Kato, Zenichiro
AU  - Kato Z
AD  - Department of Pediatrics, Gifu University School of Medicine, Tsukasa 40, Gifu 
      500-8705, Japan. zen-k@cc.gifu-u.ac.jp
FAU - Jee, JunGoo
AU  - Jee J
FAU - Shikano, Hiroaki
AU  - Shikano H
FAU - Mishima, Masaki
AU  - Mishima M
FAU - Ohki, Izuru
AU  - Ohki I
FAU - Ohnishi, Hidenori
AU  - Ohnishi H
FAU - Li, Ailian
AU  - Li A
FAU - Hashimoto, Kazuyuki
AU  - Hashimoto K
FAU - Matsukuma, Eiji
AU  - Matsukuma E
FAU - Omoya, Kentaro
AU  - Omoya K
FAU - Yamamoto, Yutaka
AU  - Yamamoto Y
FAU - Yoneda, Teruyo
AU  - Yoneda T
FAU - Hara, Takane
AU  - Hara T
FAU - Kondo, Naomi
AU  - Kondo N
FAU - Shirakawa, Masahiro
AU  - Shirakawa M
LA  - eng
SI  - PDB/1ITB
SI  - PDB/1JOS
SI  - PDB/2I1B
PT  - Journal Article
DEP - 20031005
PL  - United States
TA  - Nat Struct Biol
JT  - Nature structural biology
JID - 9421566
RN  - 0 (IL18R1 protein, human)
RN  - 0 (Interleukin-18)
RN  - 0 (Interleukin-18 Receptor alpha Subunit)
RN  - 0 (Receptors, Interleukin)
RN  - 0 (Receptors, Interleukin-18)
SB  - IM
MH  - Amino Acid Sequence
MH  - Binding Sites
MH  - Humans
MH  - Interleukin-18/*chemistry/*metabolism
MH  - Interleukin-18 Receptor alpha Subunit
MH  - Molecular Sequence Data
MH  - Receptors, Interleukin/metabolism
MH  - Receptors, Interleukin-18
EDAT- 2003/10/07 05:00
MHDA- 2003/12/25 05:00
CRDT- 2003/10/07 05:00
PHST- 2002/12/17 00:00 [received]
PHST- 2003/08/13 00:00 [accepted]
PHST- 2003/10/07 05:00 [pubmed]
PHST- 2003/12/25 05:00 [medline]
PHST- 2003/10/07 05:00 [entrez]
AID - nsb993 [pii]
AID - 10.1038/nsb993 [doi]
PST - ppublish
SO  - Nat Struct Biol. 2003 Nov;10(11):966-71. doi: 10.1038/nsb993. Epub 2003 Oct 5.