PMID- 14529020
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20031106
LR  - 20190823
IS  - 0003-2654 (Print)
IS  - 0003-2654 (Linking)
VI  - 128
IP  - 9
DP  - 2003 Sep
TI  - PDMS-based microfluidic device with multi-height structures fabricated by 
      single-step photolithography using printed circuit board as masters.
PG  - 1137-42
AB  - We have developed a method for fabricating microfluidic devices with multi-height 
      structures using single step photolithography. The whole fabrication process is 
      executed by conventional printed circuit board (PCB) technology without the need 
      of having access to clean room facilities. Specifically designed "windows" and 
      "rims" architectures were printed on films that were used as photomasks. 
      Different levels of protruding features on the PCB master were produced by 
      exposing a photomask followed by chemical wet etching. Poly(dimethylsiloxane) 
      (PDMS) was then moulded against the positive relief master to generate 
      microfluidic structures. In this report, we described the fabrication of a 
      microfluidic device featured with a multi-height "sandbag" structure for particle 
      entrapment and peripheral microchannels. Controlled immobilization of biological 
      cells and immunocytochemcial staining assays were performed to demonstrate the 
      applicability of the microfluidic device for cellular analysis. The integrity of 
      the microdevice remained stable under applied pressure, indicating the robustness 
      of the elastic PDMS structures for analytical operation. The simple 
      microfabrication process requires only low-cost materials and minimal specialized 
      equipment and can reproducibly produce mask lines of about 20 microm in width, 
      which is sufficient for most microfluidic applications.
FAU - Li, Cheuk-Wing
AU  - Li CW
AD  - Department of Biology and Chemistry, City University of Hong Kong, 83 Tat Chee 
      Avenue, Kowloon, Hong Kong, China.
FAU - Cheung, Chung Nam
AU  - Cheung CN
FAU - Yang, Jun
AU  - Yang J
FAU - Tzang, Chi Hung
AU  - Tzang CH
FAU - Yang, Mengsu
AU  - Yang M
LA  - eng
PT  - Journal Article
PL  - England
TA  - Analyst
JT  - The Analyst
JID - 0372652
EDAT- 2003/10/08 05:00
MHDA- 2003/10/08 05:01
CRDT- 2003/10/08 05:00
PHST- 2003/10/08 05:00 [pubmed]
PHST- 2003/10/08 05:01 [medline]
PHST- 2003/10/08 05:00 [entrez]
AID - 10.1039/b304354a [doi]
PST - ppublish
SO  - Analyst. 2003 Sep;128(9):1137-42. doi: 10.1039/b304354a.