PMID- 14529317
OWN - NLM
STAT- MEDLINE
DCOM- 20050908
LR  - 20191111
IS  - 1084-8592 (Print)
IS  - 1084-8592 (Linking)
VI  - 7
IP  - 1
DP  - 2003
TI  - Detection of ALK gene rearrangements in formalin-fixed, paraffin-embedded tissue 
      using a fluorescence in situ hybridization (FISH) probe: a search for optimum 
      conditions of tissue archiving and preparation for FISH.
PG  - 27-33
AB  - BACKGROUND: It is widely known that the efficiency of fluorescence in situ 
      hybridization (FISH) probes applied to formalin-fixed, paraffin-embedded tissues 
      is affected by the conditions under which the tissues are fixed and embedded. 
      However, relatively few studies address exactly how tissue archiving conditions 
      affect the performance of FISH probes. We report our experience based on use of 
      an ALK FISH probe, during the validation of its diagnostic utility. METHODS: We 
      applied the probe to 77 formalin-fixed, paraffin-embedded tissue blocks archived 
      from 1991 through to 2000, and studied the interrelationship between the archival 
      age (which ranged up to 10 years), type and condition of tissue, duration 
      required for optimum hydrolysis, and obtainability of hybridization signals. 
      RESULTS: We found that as archival age and tissue collagen content increased, not 
      only did hydrolysis times have to be prolonged in order to yield interpretable 
      hybridization signals, but also the likelihood of blocks becoming non-signaling 
      increased. The most striking positive correlations were seen between the archival 
      age of signaling lymphoid blocks and their requisite hydrolysis times. 
      CONCLUSIONS: The difficulty in applying FISH on archival tissue increases with 
      its archival age and collagen content, and may necessitate changes in laboratory 
      protocol accordingly.
FAU - Tan, Leonard Hwan Cheong
AU  - Tan LH
AD  - Department of Pathology, National University of Singapore, Singapore. 
      pattanl@nus.edu.sg
FAU - Do, Elaine
AU  - Do E
FAU - Chong, Siew Meng
AU  - Chong SM
FAU - Koay, Evelyn Siew Chuan
AU  - Koay ES
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Diagn
JT  - Molecular diagnosis : a journal devoted to the understanding of human disease 
      through the clinical application of molecular biology
JID - 9614965
RN  - 0 (Fixatives)
RN  - 1HG84L3525 (Formaldehyde)
RN  - 9007-34-5 (Collagen)
RN  - EC 2.7.10.1 (ALK protein, human)
RN  - EC 2.7.10.1 (Anaplastic Lymphoma Kinase)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
SB  - IM
MH  - Anaplastic Lymphoma Kinase
MH  - Collagen/analysis
MH  - Fixatives
MH  - Formaldehyde
MH  - *Gene Rearrangement
MH  - Humans
MH  - Hydrolysis
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Lymphoid Tissue/pathology
MH  - Neoplasms/chemistry/pathology
MH  - Paraffin Embedding
MH  - Protein-Tyrosine Kinases/*genetics
MH  - Receptor Protein-Tyrosine Kinases
MH  - *Specimen Handling
MH  - Time Factors
EDAT- 2003/10/08 05:00
MHDA- 2005/09/09 09:00
CRDT- 2003/10/08 05:00
PHST- 2003/10/08 05:00 [pubmed]
PHST- 2005/09/09 09:00 [medline]
PHST- 2003/10/08 05:00 [entrez]
AID - 715 [pii]
AID - 10.1007/BF03260017 [doi]
PST - ppublish
SO  - Mol Diagn. 2003;7(1):27-33. doi: 10.1007/BF03260017.