PMID- 14531428
OWN - NLM
STAT- MEDLINE
DCOM- 20031216
LR  - 20131121
IS  - 0273-1223 (Print)
IS  - 0273-1223 (Linking)
VI  - 48
IP  - 4
DP  - 2003
TI  - Investigation of variations in microbial diversity in anaerobic reactors treating 
      landfill leachate.
PG  - 105-12
AB  - A young landfill leachate containing elevated total ammonia concentrations 
      frequently as high as 2,700 mg/l was anaerobically treated for 1,015 days in two 
      different laboratory-scale anaerobic reactors configured as sludge blanket and 
      hybrid bed. In this paper, the last 265 days of this long-term anaerobic 
      treatability study are presented. Effects of high ammonia concentrations on 
      reactor performances were correlated to the variations in microbial diversity by 
      identifying the dominant microorganisms with FISH (fluorescent in-situ 
      hybridization), cloning, DGGE (denaturing gradient gel electrophoresis) and 
      morphological analysis. The results have indicated that the high ammonia landfill 
      leachate can be treated successfully by using either an UASB or a hybrid bed 
      reactor if temporary pH adjustments in the reactor influents are made when high 
      ammonia concentrations are experienced. Consequently, COD removal efficiency is 
      independent of microbial diversity and reactor configuration and it depends on 
      the biodegradable portion of the leachate. Under this circumstance, stability of 
      the reactors with low levels of acetate was supported by the abundance of 
      Methanosaeta population. In both of the reactors, some populations of 
      Methanobacteriaceae were also detected while other methanogenic species were 
      virtually absent. However, after the termination of pH adjustment at Day 860, 
      reactors immediately became unstable due to the sudden increase in free ammonia 
      concentration up to 400 mg/l. COD removal efficiency decreased to 42% in the 
      hybrid bed and to 48% in the UASB reactor. The durations of inhibitions were not 
      long enough to severely deteriorate the massive Methanosaeta cells; therefore, 
      many of them were again identified after two free ammonia inhibitions. However, 
      subsequently, long filamentous morphologies of Methanosaeta cells shifted to 
      shorter filaments and they lost their aggregating property.
FAU - Calli, B
AU  - Calli B
AD  - Department of Environmental Engineering, Faculty of Engineering, Marmara 
      University, 81040 Goztepe, Istanbul, Turkey. bcalli@eng.marmara.edu.tr
FAU - Mertoglu, B
AU  - Mertoglu B
FAU - Tas, N
AU  - Tas N
FAU - Inanc, B
AU  - Inanc B
FAU - Yenigun, O
AU  - Yenigun O
FAU - Ozturk, I
AU  - Ozturk I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Water Sci Technol
JT  - Water science and technology : a journal of the International Association on 
      Water Pollution Research
JID - 9879497
RN  - 0 (DNA, Bacterial)
RN  - 7664-41-7 (Ammonia)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Ammonia/analysis
MH  - *Bacteria, Anaerobic
MH  - *Bioreactors
MH  - DNA, Bacterial/analysis
MH  - Hydrogen-Ion Concentration
MH  - In Situ Hybridization, Fluorescence
MH  - *Methanobacteriaceae
MH  - Oxygen/analysis
MH  - Population Dynamics
MH  - *Refuse Disposal
EDAT- 2003/10/09 05:00
MHDA- 2003/12/17 05:00
CRDT- 2003/10/09 05:00
PHST- 2003/10/09 05:00 [pubmed]
PHST- 2003/12/17 05:00 [medline]
PHST- 2003/10/09 05:00 [entrez]
PST - ppublish
SO  - Water Sci Technol. 2003;48(4):105-12.