PMID- 14532907
OWN - NLM
STAT- MEDLINE
DCOM- 20031117
LR  - 20200409
IS  - 0022-2143 (Print)
IS  - 1532-6543 (Electronic)
IS  - 0022-2143 (Linking)
VI  - 142
IP  - 3
DP  - 2003 Sep
TI  - Experimental hypersensitivity pneumonitis: role of MCP-1.
PG  - 187-95
AB  - Inhalation of Saccharopolyspora rectivirgula causes "farmer's lung" disease, a 
      classic example of hypersensitivity pneumonitis (HP). Monocyte chemoattractant 
      protein-1 (MCP-1) is increased in the bronchoalveolar lavage fluid of mice 
      challenged with S rectivirgula, and S rectivirgula induces MCP-1 secretion by 
      alveolar macrophages. We tested the hypothesis that MCP-1 and its receptor CC 
      chemokine receptor-2 (CCR2) are essential to the development of experimental HP 
      by treating mice with MCP-1 antibody and using CCR2(-/-) mice. Administration of 
      anti-MCP-1 did not change the response to intratracheally administered S 
      rectivirgula. CCR2(-/-) animals responded in a fashion similar to that of 
      wild-type animals to intratracheally administered.S rectivirgula. To determine 
      the influence of the MCP-1-CCR2 interaction in vitro, we transferred S 
      rectivirgula-cultured spleen cells from S rectivirgula-sensitized mice, to naive 
      recipients. Later, challenge of the recipients with intratracheal S rectivirgula 
      and examination of both lung histology and bronchoalveolar lavage fluid 
      characteristics were used to determine whether adoptive transfer had occurred. We 
      found that cultured cells from CCR2(-/-) animals were fully capable of adoptive 
      transfer. We conclude that interaction of MCP-1 with CCR2 is not necessary for 
      the development of pulmonary inflammation in response to intratracheally 
      administered S rectivirgula or cells able to adoptively transfer experimental HP.
FAU - Schuyler, Mark
AU  - Schuyler M
AD  - Department of Medicine, Albequerque Veterans Affairs Medical Center, University 
      of New Mexico, 87108, USA.
FAU - Gott, Katherine
AU  - Gott K
FAU - Cherne, Amy
AU  - Cherne A
LA  - eng
GR  - HL44253/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Lab Clin Med
JT  - The Journal of laboratory and clinical medicine
JID - 0375375
RN  - 0 (Antibodies)
RN  - 0 (Ccr2 protein, mouse)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Receptors, CCR2)
RN  - 0 (Receptors, Chemokine)
SB  - IM
MH  - Adoptive Transfer
MH  - Alveolitis, Extrinsic Allergic/*immunology/*metabolism/microbiology
MH  - Animals
MH  - Antibodies/pharmacology
MH  - Chemokine CCL2/immunology/*metabolism
MH  - Farmer's Lung/immunology/metabolism/microbiology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Mutant Strains
MH  - Receptors, CCR2
MH  - Receptors, Chemokine/*genetics/metabolism
MH  - Saccharopolyspora
MH  - Spleen/cytology
PMC - PMC7126991
EDAT- 2003/10/09 05:00
MHDA- 2003/12/03 05:00
PMCR- 2003/10/04
CRDT- 2003/10/09 05:00
PHST- 2003/10/09 05:00 [pubmed]
PHST- 2003/12/03 05:00 [medline]
PHST- 2003/10/09 05:00 [entrez]
PHST- 2003/10/04 00:00 [pmc-release]
AID - S0022214303001070 [pii]
AID - S0022-2143(03)00107-0 [pii]
AID - 10.1016/S0022-2143(03)00107-0 [doi]
PST - ppublish
SO  - J Lab Clin Med. 2003 Sep;142(3):187-95. doi: 10.1016/S0022-2143(03)00107-0.