PMID- 14534793
OWN - NLM
STAT- MEDLINE
DCOM- 20040322
LR  - 20181113
IS  - 1435-2443 (Print)
IS  - 1435-2443 (Linking)
VI  - 388
IP  - 5
DP  - 2003 Oct
TI  - The role of cell type in bone healing mediated by ex vivo gene therapy.
PG  - 347-55
AB  - BACKGROUND: The ideal cellular vehicle for use in cell-mediated gene therapy to 
      enhance bone healing has not yet been identified. The purpose of this study was 
      to compare the capacity of two types of cells transduced with retro-bone 
      morphogenetic protein 4 (BMP4)-muscle-derived cells (MDCs) and unfractioned bone 
      marrow stromal cells (BMSCs). METHOD: Primary rat MDCs and unfractioned rat BMSCs 
      were transduced with a retrovirus to express BMP4. A 7-mm, critical-sized femur 
      defect was created in adult rats, and 5 x 10(6) transduced cells were implanted 
      into the femoral defect. Bone healing was monitored radiographically and 
      histologically at 4, 8, and 12 weeks post-implantation. RESULTS: All specimens in 
      the MDC-BMP4 group and BMSC-BMP4 group showed a bridging callus at 8 and 12 
      weeks. At 12 weeks post-implantation the calluses of the MDC-BMP4 femora 
      displayed significantly higher bone photodensity than the BMSC-BMP4 femora 
      (P<0.05). Histomorphometry revealed no difference between the two treatment 
      groups. However, non-union between newly formed and original bone was observed in 
      none of the MDC femora but in six femora from the BMSC-BMP4 group. CONCLUSION: 
      Both MDCs and unfractioned BMSCs can improve healing of a critical-sized bone 
      defect following transduction of the cells with retroBMP4. However, MDCs appear 
      to yield superior results when compared with BMSCs in terms of improved healing 
      of segmental defects.
FAU - Rose, Tim
AU  - Rose T
AD  - Department of Trauma and Reconstructive Surgery, University of Leipzig, 
      Liebigstrasse 20a, 04103 Leipzig, Germany. rost@medizin.uni-leipzig.de
FAU - Peng, Hairong
AU  - Peng H
FAU - Shen, Hsain-Chung
AU  - Shen HC
FAU - Usas, Arvydas
AU  - Usas A
FAU - Kuroda, Ryosuke
AU  - Kuroda R
FAU - Lill, Helmut
AU  - Lill H
FAU - Fu, Freddie H
AU  - Fu FH
FAU - Huard, Johnny
AU  - Huard J
LA  - eng
PT  - Journal Article
DEP - 20031008
PL  - Germany
TA  - Langenbecks Arch Surg
JT  - Langenbeck's archives of surgery
JID - 9808285
RN  - 0 (Bmp4 protein, rat)
RN  - 0 (Bone Morphogenetic Protein 4)
RN  - 0 (Bone Morphogenetic Proteins)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/*physiology
MH  - Bone Morphogenetic Protein 4
MH  - Bone Morphogenetic Proteins/metabolism
MH  - Chondrocytes/pathology
MH  - *Genetic Therapy
MH  - Male
MH  - Rats
MH  - Rats, Inbred F344
MH  - Stromal Cells/physiology
MH  - Transduction, Genetic
MH  - Wound Healing/*physiology
EDAT- 2003/10/10 05:00
MHDA- 2004/03/23 05:00
CRDT- 2003/10/10 05:00
PHST- 2003/04/01 00:00 [received]
PHST- 2003/06/17 00:00 [accepted]
PHST- 2003/10/10 05:00 [pubmed]
PHST- 2004/03/23 05:00 [medline]
PHST- 2003/10/10 05:00 [entrez]
AID - 10.1007/s00423-003-0401-7 [doi]
PST - ppublish
SO  - Langenbecks Arch Surg. 2003 Oct;388(5):347-55. doi: 10.1007/s00423-003-0401-7. 
      Epub 2003 Oct 8.